Effects of rhGLP-1 (7-36) on Akt/GSK3 signaling pathway in hepatocytes
10.3760/cma.j.cn.115807-20220801-00209-1
- VernacularTitle:rhGLP-1（7-36）对肝细胞Akt/GSK3信号通路的影响
- Author:
Zhiying BIAN
1
;
Yongqing ZHANG
;
Hongyu WANG
;
Fei GAO
;
Yao YAO
;
Yi WANG
;
Boyu ZHU
;
Chun XU
Author Information
1. 解放军总医院第三医学中心内分泌科，北京 100039
- Keywords:
Incretin;
Akt;
Insulin signaling pathway;
Human liver HL7702 cell line
- From:
Chinese Journal of Endocrine Surgery
2023;17(3):291-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of rhGLP-1 (7-36) on Akt/GSK3 signaling pathway in hepatocytes.Methods:Human HL7702 cell line was cultured to the logarithmic growth stage and divided into experimental group and blank control group. The cultures were incubated with 100nM medium containing rhglp-1 (7-36) and without rhglp-1 (7-36) for 90min. The levels of Akt, Glycogen synthase Kinase 3 (GSK3) and Glycogen synthase (GS) in the two groups were detected by Western Blot.Results:Compared with blank control group, the protein expression of p-Akt (Thr308) in experimental group (1.81±0.28) was significantly increased ( P=0.01), but the protein expression of Akt and p-Akt (Ser473) was not significantly changed. The protein expression levels of p-GSK3α (Ser21) (1.27±0.09) and p-GSK3β (Ser9) (1.24±0.09) in the experimental group were significantly increased ( P=0.003, 0.002), while the protein expression levels of GSK3α and GSK3β were not significantly changed. The protein expression level of p-GS (Ser641) (0.70±0.16) was decreased in the experimental group ( P=0.03), but the protein expression level of GS did not change significantly. Conclusion:Glp-1 can inhibit GSK3/GS signaling pathway, activate GS activity and promote glycogen synthesis.
