Rab25 gene down-regulates snail expression and inhibits epithelial mesenchymal transformation of human breast cancer MDA-MB-231 cells
10.3760/cma.j.cn.115807-20230115-00016
- VernacularTitle:Rab25基因下调snail表达抑制人乳腺癌MDA-MB-231细胞上皮-间充质转化能力的研究
- Author:
Xiaobin ZHANG
1
;
Yunfei LU
;
Xiaolong GUI
;
Jiehua LI
Author Information
1. 广西医科大学第一附属医院胃肠腺体外科，南宁 530021
- Keywords:
Rab25;
Breast cancer;
Epithelial mesenchymal transformation;
TGF-β;
Snail protein;
AKT signal path way
- From:
Chinese Journal of Endocrine Surgery
2023;17(2):166-169
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To probe into Rab25 Gene’s Effect on TGF-β inhibition of proliferation, invasion and epithelial mesenchymal transformation (EMT) of breast cancer MDA-MB-231 cells and explore its molecular mechanism.Methods:The experiment was divided into three groups: control group,TGF-β Group and si-Rab25 group. TGF-β induced MDA-MB-231 cell model of EMT was built. CCK-8 assay was used to detect cell proliferation. Transwell assay was used to detect the ability of cell invasion and migration.Western blot was used to detect the changes of related proteins in each group.Results:After stimulating MDA-MB-231 cells with TGF-β, Rab25 gene was highly expressed. Compared with TGF-β group (57.48±%3.62%), the migration ability and invasion ability of cells in si-Rab25 group (33.49%±2.93%) decreased by 41.7%, with a significant difference ( P<0.05). Compared with TGF-β group (153.21%±4.17%), the proliferation ability of cells in si-Rab25 group (115.32%±5.69%) decreased by 24.73%, with a significant difference ( P<0.05). The expression of MDA-MB-231 fine EMT related protein in si-Rab25 group was significantly different from that in TGF-β group ( P<0.05). The expression of p-AKT and Snail protein in si-Rab25 group was significantly lower than that in TGF-β group ( P<0.05) . Conclusions:Rab25 gene is highly expressed in MDA-MB-231 cells. Silencing Rab25 gene can activate AKT signal pathway, inhibit Snail protein expression, regulate EMT related protein expression, and inhibit EMT transformation.
