Altered expression of gamma-secretase components in animal model of major depressive disorder induced by reserpine administration.
10.5625/lar.2012.28.2.109
- Author:
Hye Ryun LEE
1
;
In Sik HWANG
;
Ji Eun KIM
;
Sun Il CHOI
;
Young Ju LEE
;
Jun Seo GOO
;
Eon Pil LEE
;
Hae Wook CHOI
;
Hong Sung KIM
;
Jae Ho LEE
;
Young Jin JUNG
;
Dae Youn HWANG
Author Information
1. Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University, Miryang, Korea. dyhwang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Major depressive disorder;
Alzheimer's disease;
reserpine;
gamma-secretase;
APP
- MeSH:
Alzheimer Disease;
Amyloid;
Amyloid Precursor Protein Secretases;
Animals;
Blotting, Western;
Brain;
Depressive Disorder, Major;
Membrane Glycoproteins;
Mice;
Mice, Inbred ICR;
Models, Animal;
Nerve Growth Factors;
Phosphorylation;
Proteins;
Reaction Time;
Reserpine
- From:Laboratory Animal Research
2012;28(2):109-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
Altered expression of neurotrophic factors as well as neuroinflammation is commonly associated with Major depressive disorder (MDD) and Alzheimer's disease (AD). To investigate whether or not reserpine-induced MDD affects the expression of AD-related proteins, the expression of gamma-secretase components and substrate were measured in brains of ICR mice following reserpine treatment for 15 days. In active avoidance test, total response time and peak slightly increased in the 2 mg/kg reserpine (RSP2)-treated group compared to vehicle-treated group (P<0.05). Expression and phosphorylation of MKP-1, which is a key factor in MDD pathology, were both higher in the RSP2-treated group than the vehicle- and 1 mg/kg reserpine (RSP1)-treated groups (P<0.02). Furthermore, full-length expression of amyloid precursor protein (APP) was enhanced in the RSP1 and RSP2-treated groups compared to the vehicle-treated group, whereas expression of gamma-secretase components decreased (P<0.03). Among the three components of the gamma-secretase complex, nicastrin protein underwent the largest decrease in expression, as detected by Western blotting (P<0.03). Therefore, the data presented here provide additional evidence about the pathological correlation between MDD and AD.
