Monitoring of antibiotic resistance in bacteria isolated from laboratory animals.
10.5625/lar.2012.28.2.141
- Author:
Jun Seo GOO
1
;
Mee Kyung JANG
;
Sun Bo SHIM
;
Seung Wan JEE
;
Su Hae LEE
;
Chang Jun BAE
;
Songhee PARK
;
Kwang Je KIM
;
Ji Eun KIM
;
In Sik HWANG
;
Hye Ryun LEE
;
Sun Il CHOI
;
Young Ju LEE
;
Chul Joo LIM
;
Dae Youn HWANG
Author Information
1. Department of Biomaterials Science, Collage of Natural Resources & Life Science, Pusan National University, Miryang, Korea. dyhwang@pusan.ac.kr
- Publication Type:Letter
- Keywords:
Drug resistance;
Enterococcus faecalis;
Acinetobacter baumannii;
oxacillin;
nitrofurantoin
- MeSH:
Acinetobacter baumannii;
Agriculture;
Animals;
Animals, Laboratory;
Anti-Bacterial Agents;
Bacteria;
Bronchi;
Cefazolin;
Cefoxitin;
Citrobacter freundii;
Drug Resistance;
Drug Resistance, Microbial;
Enterococcus faecalis;
Escherichia coli;
Intestines;
Korea;
Mice;
Mice, Inbred ICR;
Nitrofurantoin;
Nose;
Oxacillin;
Pharmacy;
Rats;
Rodentia;
Sphingomonas;
Staphylococcus aureus;
Staphylococcus epidermidis;
Veterinary Medicine
- From:Laboratory Animal Research
2012;28(2):141-145
- CountryRepublic of Korea
- Language:English
-
Abstract:
The drug resistance of microorganisms isolated from laboratory animals never treated with antibiotics is being reported consistently, while the number of laboratory animals used in medicine, pharmacy, veterinary medicine, agriculture, nutrition, and environmental and health science has increased rapidly in Korea. Therefore, this study examined the development of antimicrobial resistance in bacteria isolated from laboratory animals bred in Korea. A total of 443 isolates (7 species) containing 5 Sphingomonas paucimobilis, 206 Escherichia coli, 60 Staphylococcus aureus, 15 Staphylococcus epidermidis, 77 Enterococcus faecalis, 27 Citrobacter freundii, 35 Acinetobacter baumannii were collected from the nose, intestine, bronchus and reproductive organs of ICR mice and SD rats. Of these species, Acinetobacter baumannii and Enterococcus faecalis showed significant antimicrobial resistance according to the minimum inhibition concentration (MIC) in E-test. In case of Acinetobacter baumannii, several isolates showed MIC values 16-128 microg/mL for cefazolin and cefoxitin, and higher resistance (128-512 microg/mL) to nitrofurantoin than that of standard type. Resistance to cefazolin, cefoxitin and nitrofurantoin was detected in 17.14, 20.00, and 8.57% of the Acinetobacter baumannii isolates, respectively. In addition, 44.1% of the Enterococcus faecalis isolates collected from the laboratory animals were resistant to oxacillin concentration of 16-32 microg/mL range, while MIC value of standard type was below oxacillin concentration of 6 microg/mL. These results suggest that in rodent species of laboratory animals, Acinetobacter baumannii are resistance to cefazolin, cefoxitin and nitrofurantoin, whereas those of Enterococcus faecalis were resistance to oxacillin.
