Determination of eight active components in Buyang Huanwu Decoction by HPLC-DAD-ELSD
10.3760/cma.j.cn115398-20220520-00270
- VernacularTitle:HPLC-DAD-ELSD法测定补阳还五汤中8个活性成分含量
- Author:
Mingcong LI
1
;
Yixin DONG
;
Yuming ZHUANG
;
Ping YU
;
Hui ZHAO
;
Haiyan ZOU
Author Information
1. 首都医科大学中医药学院 中医络病研究北京市重点实验室，北京 100069
- Keywords:
Bu Yang Huan Wu Tang;
Assay (TCD);
High performance liquid chromatography-secondary array detector-evaporative light scattering detector;
Active constitu
- From:
International Journal of Traditional Chinese Medicine
2023;45(6):719-724
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC-DAD-ELSD method for the simultaneous determination of eight main active components in Buyang Huanwu Decoction, including hydroxysafflor yellow A, paeoniflorin, calycosin glycoside, ferulic acid, ononin, calycosin, fermononetin and astragaloside.Methods:Agilent Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) was used with acetonitrile-0.1% formic acid as the mobile phase. The flow rate was 1.0 ml/min; the column temperature was 30 ℃; the detection wavelengths were 230 nm (paeoniflorin), 254 nm (calycosin glycoside, ononin, calycosin, fermononetin), 322 nm (ferulic acid) and 403 nm (hydroxysafflor yellow A); the drift tube temperature of the evaporative light scattering detector was 60 ℃; the carrier gas flow rate was 1.6 L/min.Results:Under these conditions, the separation of hydroxysafflor yellow A, paeoniflorin, calycosin glycoside, ferulic acid, ononin, calycosin, fermononetin and astragaloside was good, and the linear relationship was in line with the requirements ( r=0.994 0-0.999 9). The average recovery was 97.8% - 101.4% ( RSD was 1.28% - 3.70%). Conclusion:The method is simple, stable and reproducible, and can be used for the quality control of Buyang Huanwu Decoction.
