Discussion on mechanism of Danggui Buxue Decoction for anti-myocardial ischemia- reperfusion injury and "treating different diseases with the same method" in ischemic stroke based on network pharmacology
10.3760/cma.j.cn115398-20220420-00274
- VernacularTitle:基于网络药理学探讨当归补血汤抗心肌缺血再灌注损伤和缺血性卒中“异病同治”的作用机制
- Author:
Jiankun CUI
1
;
Xichun HUANG
;
Zeji NIU
;
Yanqi SHAO
;
Yuanting MEI
;
Yiyan YANG
;
Yan WANG
Author Information
1. 黑龙江中医药大学附属第一医院心血管病科，哈尔滨 150040
- Keywords:
Dang Gui Bu Xue Tang;
Myocardial reperfusion injury;
Ischemic stroke;
Network pharmacology;
Molecular docking simulation
- From:
International Journal of Traditional Chinese Medicine
2023;45(5):612-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To predict the mechanism of Danggui Buxue Decoction for anti-myocardial ischemia-reperfusion injury and "treating different diseases with the same method" in ischemic stroke based on network pharmacology and molecular docking.Methods:The active components and targets of Danggui Buxue Decoction were screened by retrieving the database of TCMSP and literature; the corresponding targets of myocardial ischemia-reperfusion injury and ischemic stroke were found by OMIM and GeneCards database; the intersection targets of Danggui Buxue Decoction and disease were obtained by using Venny diagram, and the common target network and protein-protein interaction network were constructed by Cytoscape 3.7.1 software and STRING database. The GO and KEGG pathways were enriched by David Database, and the Bio GPS database was used to obtain the tissue distribution information of the key targets. The molecular docking technology was used to verify the results.Results:There were 21 active components in Danggui Buxue Decoction, 181 effective targets and 93 cross targets with diseases. The key components were quercetin, Kaempferol, β-sitosterol, formononetin and isorhamnetin. The key targets were AKT1, TNF, IL6, IL-1β and VEGFA. The enrichment results showed that the main action pathways were fluid shear force and arteriosclerosis, lipid and arteriosclerosis, AGE-RAGE signal pathway in diabetic complications, and the core targets were mainly located in the medullary cells, dendritic cell, smooth muscle, prostate, thyroid and other tissues. The results of molecular docking showed that quercetin had the best binding effect to IL-1β, while isorhamnetin had the best binding effect to IL-1β.Conclusion:Danggui Buxue Decoction is against myocardial ischemia-reperfusion injury and ischemic stroke through hemodynamics, lipid metabolism, inflammatory reaction, oxidative stress, immune reaction and cell apoptosis, plays the role of "treating different diseases with the same method".
