Determination of 9 components Simultaneously in Swertia chirayita by HPLC method
10.3760/cma.j.cn115398-20220514-00202
- VernacularTitle:高效液相色谱法同时测定藏药印度獐牙菜中9个成分
- Author:
Yuan SU
1
;
Zengliang YANG
;
Anping LIU
;
Xueliang LIU
;
Haiqing LIU
;
Kaixiang WANG
;
Chunlan SHI
;
Weiye LI
;
Wensheng XU
;
Cunsheng ZOU
Author Information
1. 西宁市食品药品检验检测中心，西宁 810007
- Keywords:
Tibetan drugs;
Assay (TCD);
Swertia chirayita;
HPLC;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2023;45(5):594-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a HPLC method for determinating 9 components simultaneously in Swertia chirayita. Methods:By useing water Sunfire C18 column (4.6 mm× 250 mm,5 μm); Gradient elution was carried out with methanol-0.05% phosphoric acid solution as mobile phase. Setting the column temperature at 30 ℃, the flow rate at 1.0 ml/min, and the detection wavelength at 254 nm.Results:9 components showed good linear relationship within the injection quality range. The recovery rates of wertiamarin, Gentiopicroside, Angelica glycosides,Mangiferin, Isolysine, Gentianoside, Diol glycoside, 8-hydroxy-1,3,5 trimethoxyketone, and Daisy leaf gentinone were 95.38%, 92.41%, 95.14%, 91.87%, 92.24%, 92.51%, 95.08%, 91.72%, 95.74% ( n=6). Conclusion:The method is simple, efficient, sensitive, accurate, economical and practical, with repeatability and stability. It could provide reference for the quality control and comprehensive utilization of Swertia chirayita.
