Study on HPLC fingerprints of Zhuanggu Guanjie Pills
10.3760/cma.j.cn115398-2022526-00352
- VernacularTitle:壮骨关节丸HPLC指纹图谱研究
- Author:
Liwen WANG
1
;
Xiaomei YUAN
;
Liyuan CHENG
;
Yue ZHANG
;
Kun ZHOU
Author Information
1. 天津中医药大学中医药研究院，天津 300193
- Keywords:
Zhuang Gu Guan Jie Wan;
High performance liquid chromatography;
Fingerprints;
Quality control;
Assay (TCD)
- From:
International Journal of Traditional Chinese Medicine
2023;45(4):446-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the high performance liquid chromatography (HPLC)fingerprints of Zhuanggu Guanjie Pills, which provided the basis for its quality evaluation.Methods:HPLC was used with Agilent Eclipse XDB-C 18 column (4.6 mm×250 mm, 5 μm);mobile phase was acetonitrile-0.1% acetic acid water; gradient elution; flow rate was 1 ml/min; column temperature was at 35 ℃; detection wavelength was 254 nm; injection volume was 10 μl. The HPLC fingerprints of 15 batches of Zhuanggu Guanjie Pills were established and similarity analysis was carried out, and the contents of 18 components were estimated. Results:In the fingerprint study, isopsoralen was used as the reference peak, 40 common peaks were marked and 18 peaks were identified and the similarity between the fingerprints of 15 batches of Zhuanggu Guanjie Pills and the control fingerprints was greater than 0.99.Conclusion:This method is easy to operate and has high accuracy, which can provide basis and reference for the quality evaluation of Zhuanggu Guanjie Pills.
