Optimization of the extraction process of Shangke Huoxue Granule by central composite design-response surface methodology
10.3760/cma.j.cn115398-20220507-00085
- VernacularTitle:星点设计-效应面法优化伤科活血颗粒提取工艺
- Author:
Runkong WANG
1
;
Liyang ZHU
;
Mingquan WU
;
Wei PENG
;
Heng HU
;
Congyang XU
;
He TU
;
Xu ZHOU
Author Information
1. 四川省骨科医院中药研究室，成都 610041
- Keywords:
Extraction;
Central composite design-response surface methodology;
Shangke Huoxue Granule;
Ferulic acid;
Paeoniflorin;
Technology improving (TCD)
- From:
International Journal of Traditional Chinese Medicine
2023;45(4):451-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction process of Shangke Huoxue Granule.Methods:Taking the factors of extraction solvent multiple, extraction time and extraction times as investigation factors, and extraction amount of ferulic acid, paeoniflorin and the ratio of extraction as comprehensive evaluation indices, one-factor experimental design and central composite design-response surface methodology were adopted to optimize the extraction process of Shangke Huoxue Granule.Results:The binomial fitting equation was Y=96.16+2.42 A+0.63 B-3.76 AB-1.57 A2-1.87 B2 ( P<0.01). The optimal extraction process parameters were confirmed to be adding 16 times of water, 64 minutes each time, twice. The deviation rates between the measured values of three verification experiments and the predicted value were 2.00%, 3.23% and 0.66%. Conclusion:The established model of central composite design-response surface methodology has high predictability and the optimized extraction process is stable and feasible.
