The protective effect and mechanism of Ophiopogonin D on LPS-induced mouse macrophages
10.3760/cma.j.cn115398-20220506-00063
- VernacularTitle:麦冬皂苷D对脂多糖诱导的小鼠巨噬细胞保护作用的研究
- Author:
Xinyi SHAO
1
;
Guangyuan HAN
;
Ruheng WEI
;
Jianzhong GAO
Author Information
1. 山西中医药大学研究生院，晋中 030619
- Keywords:
Inflammation;
Oxidative stress;
Ophiopogonin D;
Macrophages;
NF-kappa B
- From:
International Journal of Traditional Chinese Medicine
2023;45(2):169-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the protective effect of Ophiopogonin D on lipopolysaccharide (LPS)-induced mouse macrophage RAW264.7 and its related mechanism.Methods:Mouse macrophage RAW264.7 cells were cultured and divided into normal control group, model group and Ophiopogonin D pretreatment group according to random number table method. The activity of Ophiopogonin D on RAW264.7 cells was detected by CCK-8 method; the protein levels of TNF-α, IL-1β, IL-6, reactive oxygen species (ROS), MDA and SOD were detected by ELISA; the protein expression of NF-κB, TLR4, NF-E2-related factor2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western Blot.Results:Compared with model group, the levels of TNF-α, IL-1β, IL-6, ROS and MDA in cell supernatant of Ophiopogonin D group were decreased ( P<0.05), and the level of SOD was increased ( P<0.05). The protein expressions of NF-κB (0.76±0.10 vs. 2.26±0.17) and TLR4 (0.98±0.09 vs. 1.74±0.19) significantly decreased ( P<0.05). The protein expressions of Nrf2 (0.85±0.03 vs. 0.54±0.03) and HO-1 (0.97±0.11 vs. 0.37±0.04) significantly increased ( P<0.05). Conclusion:Ophiopogonin D may reduce inflammatory response by reducing TLR4/NF-κB pathway, and activate Nrf2/HO-1 pathway to reduce oxidative damage and play a protective role.
