Content determinnation of chlorogenic acid and linarin in Yejuhua Granules
10.3760/cma.j.cn115398-20220820-00216
- VernacularTitle:HPLC法同时测定野菊花颗粒中绿原酸和蒙花苷含量
- Author:
Weiguang SUN
1
;
Manling DU
;
Ji WANG
;
Zhiyun HUANG
;
Anfeng WAN
;
Jiansheng GAO
;
Xiaotian ZHONG
Author Information
1. 广州白云山星群（药业）股份有限公司，广州 510288
- Keywords:
Assay (TCD);
Yejuhua Granules;
Chlorogenic acid;
Buddleoside;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2023;45(2):197-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determintation of chlorogenic acid and linarin in Yejuhua granules by HPLC.Methods:We applied HPLC methods. The Kromasil 100-5 C18 column (250 mm×4.6 mm,5 μm) was used, the mobile phase was acetonitrile-0.4%H 3PO 4 solution (gradient elution), the flow rate was 1.0 ml/min, the dection wavelenghth was 334 nm and the column temperture was 32 ℃. Results:Chlorogenic acid and buddleoside had good linearity in the ranges of 0.30-1.50 μg ( r2=0.999 1) and 0.12-0.62 μg ( r2=0.999 8), respectively. The average recoveries were 99.70% and 96.67%, with RSD<2%, respectively. Conclusion:The method is simple, rapid, reliable, efficient, and can be used for determination of chlorogenic acid and buddleoside in Yejuhua Granules.
