Evolutionary analysis of the VP1 gene of Echovirus 30 in Yunnan Province, China
10.3760/cma.j.cn121382-20230427-00404
- VernacularTitle:云南省埃可病毒30型VP1基因进化分析
- Author:
Yihui CAO
1
;
Lili JIANG
;
Jinghui YANG
;
Nan LI
;
Xi YANG
;
Xiaofang ZHOU
;
Jianping CUN
Author Information
1. 云南省疾病预防控制中心，昆明 650022
- Keywords:
Echovirus 30;
Genetic characterization;
Evolutionary analysis
- From:
International Journal of Biomedical Engineering
2023;46(4):306-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the genetic characteristics and genetic evolution of echovirus 30 (ECHO30) isolates in Yunnan Province, China.Methods:Virus isolation was performed on nucleic acid-positive samples for hand, foot, and mouth disease pathogen surveillance in Yunnan Province, and VP1 gene sequencing was performed. The sequences of eight ECHO30 isolates from Yunnan Province and the gene sequences of the VP1 region of the ECHO30 reference strain downloaded from GenBank were compared and analyzed using MEGA 5.0 software, and then a phylogenetic tree was constructed to measure the homology of nucleotides and amino acids between the isolates.Results:The ECHO30 virus was distributed in Wenshan, Qujing, Chuxiong, and Kunming in Yunnan Province. The ECHO30 virus was relatively common in Wenshan. ECHO30 isolates belonged to the H2 subtype of the H genotype, which was close to the local reference strain LC120939 in Yunnan Province. On the VP1 gene at site 5, the amino acid change ratio was more active, the amino acids were diverse, and mutations also occurred at sites 54, 156, 258, and so on. Nucleotide and amino acid homology were 84.0% - 100.0% and 98.4% - 100.0%, respectively.Conclusions:ECHO30 isolates from Yunnan Province have certain geographical characteristics and belong to H2 of the H genotype. The nucleotide differences in virus sequences among subtypes are small and have a close genetic relationship.
