Microfluidics-based study on the damage pattern of red blood cells and VWF
10.3760/cma.j.cn121382-20221222-00108
- VernacularTitle:基于微流控技术的红细胞与VWF损伤规律研究
- Author:
Nana HUAN
1
;
Xu MEI
;
Qiubo JIANG
;
Liudi ZHANG
Author Information
1. 苏州大学机电工程学院，生物制造研究中心，人工器官研究所，苏州 215006
- Keywords:
Microfluidics;
Erythrocytes;
Von willebrand factor;
Shear stress;
Exposure time
- From:
International Journal of Biomedical Engineering
2023;46(1):42-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of shear stress magnitude and exposure time on the damage of blood component erythrocytes and von willebrand factor (VWF) based on microfluidic technology.Methods:A blood shear platform was built based on a microfluidic chip, samples were prepared under different shear stress magnitudes and exposure time lengths, free hemoglobin assay experiments were performed on blood samples, the hemolysis indices of different samples were measured, and the relative molecular masses of different samples of VWF were analyzed by immunoblotting and chemiluminescence imaging.Results:The quantitative relationships between the hemolysis index and the degradation rate of high relative molecular mass VWF with shear stress and exposure time followed the power function model well.Conclusions:The microfluidic experimental platform has the advantages of a precise and controllable internal microenvironment and easy and rapid detection, which can be used for the quantitative study of blood damage patterns.
