Naringin alleviates early brain injury in rats with subarachnoid hemorrhage
10.3760/cma.j.cn121382-20220617-00602
- VernacularTitle:柚皮苷减轻蛛网膜下腔出血大鼠早期脑损伤的研究
- Author:
Yuwei HAN
1
;
Chenchen WANG
;
Guobiao LIANG
;
Xiaoming LI
Author Information
1. 北部战区总医院神经外科，沈阳 110016
- Keywords:
Naringin;
Subarachnoid hemorrhage;
PARP-1;
AIF;
PAR
- From:
International Journal of Biomedical Engineering
2022;45(6):479-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of naringin on early brain injury in rats with subarachnoid hemorrhage and its possible mechanism of action.Methods:Rats were randomly divided into the sham operation group, the model group, and the naringin group. Each group had 8 rats. The SAH model was established by intravascular perforation, and then rats in the model group and the naringin group were administered 0.9% NaCl or naringin 40 mg/kg by intraperitoneal injection after 0.5 h. SAH score, neurological function score, cerebral edema, and blood-brain barrier permeability were detected. The level of NAD + and nflammatory factors were detected by ELISA. The expression of poly(ADP-ribose) polymerase-1 (PARP-1), apoptosis inducing factor (AIF), and protease-activated receptor (PAR) proteins was detected by Western Blot. The expression of PARP-1 mRNA was detected by quantitative real-time fluorescence PCR (qRT-PCR). Neuronal apoptosis was detected by an immunofluorescence assay. Results:Compared with the model group, naringin treatment improved neurological function ( P<0.01), reduced cerebral edema and Evans blue exudation (all P<0.01), increased the content of NAD + ( P<0.001), reduced IL-1β, IL-6 and TNF-α levels (all P<0.001), and reduced the expression of PARP-1/AIF pathway-related proteins in vivo (all P<0.001). In addition, naringin could inhibit neuronal apoptosis in early brain injury after SAH. Conclusions:Naringin can improve the early brain injury after SAH, which may be achieved by inhibiting the PARP-1/AIF pathway.
