Establishment of gene diagnosis model of diabetic kidney disease based on bioinformatics
10.3760/cma.j.cn121382-20220728-00504
- VernacularTitle:基于生物信息学的糖尿病肾脏疾病基因诊断模型的建立
- Author:
Hua XU
1
;
Jia CHEN
;
Yuyin SONG
Author Information
1. 盘锦辽油宝石花医院检验科，盘锦 124010
- Keywords:
Diabetic kidney disease;
Biomarkers;
Bioinformatics;
Genetic diagnostic models;
Glomerular filtration rate
- From:
International Journal of Biomedical Engineering
2022;45(5):395-402,423
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential biomarkers and pathogenesis of diabetic kidney disease (DKD) using bioinformatics techniques.Methods:Three DKD glomerular tissue datasets, GSE30528, GSE96804, and GSE104948, were downloaded from the gene expression database. The bioinformatics techniques, combined with machine learning, were applied to screen DKD biomarkers and establish a DKD gene diagnostic model. The CIBERSORT algorithm was applied to analyze immune cell infiltration in DKD glomerular tissue. The correlation between biomarkers and immune cells, model risk scores, and the glomerular filtration rate (eGFR) of patients was investigated.Results:A three-gene model consisting of G6PC, CDH10 and TPPP3 was screened and established from 26 differentially expressed genes, which showed good diagnostic efficacy in both the training set (AUC=0.984) and validation set (AUC=0.992). Analysis of infiltrating immune cells in DKD glomerular tissue showed that γδ T cells, activated NK cells, M2 macrophages, resting dendritic cells, resting mast cells, activated mast cells, and neutrophils may be involved in the DKD process, and that G6PC, CDH10 and TPPP3 were associated with multiple immune cell infiltrations. Using the model to calculate the risk score of DKD patients, the higher the score, the lower the eGFR level, and the risk score was significantly negatively correlated with the eGFR level. Conclusions:G6PC, CDH10 and TPPP3 can be good diagnostic markers for DKD, correlate with DKD immune cell infiltration, and be used as targets for DKD diagnosis and treatment.
