Role of galectin-3 in the early brain injury of mice with subarachnoid hemorrhage is associated with the regulation of microglia polarization
10.3760/cma.j.issn.1673-4165.2023.05.006
- VernacularTitle:半乳糖凝集素-3在蛛网膜下腔出血小鼠早期脑损伤中的作用与调节小胶质细胞极化相关
- Author:
Yuwei HAN
1
;
Chenchen WANG
;
Guobiao LIANG
;
Xiaoming LI
Author Information
1. 北部战区总医院神经外科，沈阳 110016
- Keywords:
Subarachnoid hemorrhage;
Brain injuries;
Galectin 3;
Microglia;
Cell polarity;
Disease models, animal;
Mice
- From:
International Journal of Cerebrovascular Diseases
2023;31(5):352-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of galectin-3 (gal-3) on microglia polarization after subarachnoid hemorrhage (SAH).Methods:C57BL/6 male adult mice were used to induce SAH or sham operation models. Gal-3 siRNA or negative control siRNA was injected into the lateral ventricle 48 h before the model was induced. After 24 h of model preparation, the SAH score, neurological function score, brain water content, and Evans blue exudate were measured. Western blot analysis was used to detect the expressions of M1 phenotypic markers (inducible nitric oxide synthase [iNOS], CD11b, tumor necrosis factor [TNF]-α) and M2 phenotype markers (CD206, YM1/2, arginase-1 [Arg1]).Results:After using Gal-3 siRNA to inhibit Gal-3, the neurological function score significantly increased, while the SAH score, brain water content, and Evans blue exudate significantly decreased ( P<0.001). Western blot analysis showed that the expressions of M1 phenotypic markers (iNOS, CD11b and TNF-α) in microglia were significantly decreased after Gal-3 inhibition, while the expressions of M2 phenotypic markers (CD206, YM1/2 and Arg1) were significantly increased ( P<0.001). Conclusion:Inhibition of Gal-3 expression can alleviate the early brain injury after SAH, and its mechanism may be associated with regulating the polarization of microglia from M1 to M2 phenotype.
