Loss of Heterozygosity of p73, APC, and p53 in Hepatoblastoma.
- Author:
Han Seong KIM
;
Young Mi JUNG
;
Mi Ran KIM
;
Jung Young LEE
;
Mi Sook LEE
;
Ja June JANG
- Publication Type:Original Article
- Keywords:
Hepatoblastoma;
loss of heterozygosity;
p73;
APC;
p53
- MeSH:
Carcinogenesis;
Hepatoblastoma*;
Liver;
Loss of Heterozygosity*
- From:The Korean Journal of Hepatology
1999;5(1):43-49
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIMS: The status of tumor suppression gene can be assessed indirectly by analyzing the loss of heterozygosity. Hepatoblastoma is a malignant liver tumor in childhood. To find the molecular carcinogenetic mechanism of hepatoblastoma, loss of heterozygosity (LOH) of p73, APC and p53 was studied. MATERIALS AND METHODS: Hepatoblastoma tissues from thirty-three cases were collected by lobectomy or tumorectomy. On H- stained sections, normal and tumor cells were microdissected separately and LOH analysis was perfomed using 8 markers: six of p73, one of APC and one of p53. RESULTS: Number of cases showing at least one LOH in six p73 markers was four out of twenty- six (15.4%): each LOH frequencies in D1S160, D1S170, D1S199, D1S228, D1S243 and D1S253 were in order of 7.7%, 0%, 9.1%, 0%, 12.5% and 0%. LOH frequency of APC was 41.7% and that of p53 was 13.3%. CONCLUSION: Low LOH frequency of p73 related markers indicates that p73 gene may not be implicated in carcinogenesis of hepatoblastoma.
