Establishment of mouse silicosis fibrosis model by non-exposed tracheal perfusion
10.20001/j.issn.2095-2619.20230203
- VernacularTitle:非暴露式气管灌注法建立小鼠矽肺纤维化模型
- Author:
Xiaoxue GONG
1
;
Lingfang FENG
;
Yongxin LI
;
Junfei CHEN
;
Xiaowen DONG
;
Jiaohui YAO
;
Jianlin LOU
Author Information
1. School of Public Health, Hangzhou Medical College, Hangzhou, Zhejiang 310013, China
- Publication Type:Journal Article
- Keywords:
Silicosis;
Mice;
Tracheal perfusion;
Lung;
Fibrosis;
Model
- From:
China Occupational Medicine
2023;50(1):17-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a convenient non-invasive tracheal perfusion method for constructing a mouse model of silicosis-induced pulmonary fibrosis. Methods: The specific pathogen-free C57BL/6 mice were randomly divided into control group and model group, with 15 mice in each group. After anesthesia, a 22G arteriovenous indwelling needle was used to inset into the trachea through the mice's mouth. The model group mice were perfused with 0.1 mL of silica suspension with a mass concentration of 25 g/L, and the mice in the control group were perfused with an equal volume of 0.9% sodium chloride solution. On the 7th, 14th, and 30th day after modeling, the body weight of the mice was measured, and the lung tissue morphology and pathological changes were observed. The expression of α-smooth muscle actin (α-SMA) and collagen type 1 alpha 1 (COL1A1) protein in lung tissue of mice was detected by immunofluorescence on the 30th day after modeling. Results: There was no death of mice in the two groups during the experiment. There was no significant difference in body weight between the two groups (P>0.05). The lung tissues of the mice in the model group were pinkish-gray and uneven in color on the 7th and 14th days after dust exposure. On the 30th day after dust exposure, the lung tissue of the mice in the model group was gray and hard, and unevenly distributed silicon nodules were visible by the naked eyes. The histopathology results of lung tissue showed that compared with the mice in control group, the model group mice exhibited persistent aggravation of pulmonary inflammation, thickening of alveolar septum, infiltration of inflammatory cells gradually clustering into clumps, and an increasing number of fibrous foci.On the 30th day after dust exposure, the relative expression of α-SMA and COL1A1 proteins in the lung of the model group was higher than those in the control group (median： 72.59 vs 5.91, 35.62 vs 10.07, both P<0.05). Conclusion: The method of tracheal perfusion silica suspension of mice using 22G arteriovenous indwelling needle can successfully construct an animal model of silicosis fibrosis. This method is convenient, safe and effective, and is worth promoting.
