Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma
10.3971/j.issn.1000-8578.2023.22.1246
- VernacularTitle:LncRNA PTENP1在TGF-β诱导的食管鳞状细胞癌上皮间质转化中的作用
- Author:
Shenbo FU
1
;
Tuotuo GONG
;
Junjun GUO
Author Information
1. Department of Radiation Oncology, Shaanxi Provincial Tumor Hospital, Xi'an 710061, China
- Publication Type:Research Article
- Keywords:
Esophageal squamous cell carcinoma;
LncRNA PTENP1;
TGF-β;
Migration;
Epithelial-mesenchymal transition
- From:
Cancer Research on Prevention and Treatment
2023;50(9):847-853
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of lncRNA PTENP1 in regulating TGF-β-induced epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC). Methods Eca109 and TE-1 cells were treated with TGF-β1, and the expression of PTENP1 was detected by qRT-PCR before and after treatment. PTENP1-overexpressing stably transfected cell lines were constructed in Eca109 and TE-1 cells. The effects of overexpression of PTENP1 on TGF-β1-induced migration, proliferation and EMT-related proteins expression in Eca109 and TE-1 cells were detected by Transwell assay, CCK-8 test and Western blot, respectively. Results The expression of PTENP1 was significantly decreased in Eca109 and TE-1 cells treated with TGF-β1 (P < 0.05). Overexpression of PTENP1 significantly prevented cell migration, decreased the cell vitality, upregulated the E-cadherin expression, and downregulated the expression of N-cadherin and vimentin in Eca109 and TE-1 cells (P < 0.05). Furthermore, PTENP1 overexpression attenuated TGF-β-induced migration of Eca109 and TE-1 cells. PTENP1 overexpression partially reversed TGF-β-induced EMT (P < 0.05). Conclusion PTENP1 plays an important role in TGF-β-induced EMT in ESCC cells.
