Effect of LncRNA GAPLINC on the Cell Proliferation of Rheumatoid Arthritis Fibroblast-like Synoviocytes
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0510
- VernacularTitle:长链非编码RNA GAPLINC对类风湿关节炎成纤维样滑膜细胞增殖表型的影响
- Author:
Bi-yao MO
1
;
Lu XIAO
1
;
Yu-wei ZHAN
1
;
Yang YANG
1
;
Li LIU
1
;
Shu-dian LIN
1
Author Information
1. Department of Rheumatology， Hainan Affiliated Hospital of Hainan Medical University// Hainan General Hospital， Haikou 570311，China
- Publication Type:Journal Article
- Keywords:
long non-coding RNA;
rheumatoid arthritis;
synovial hyperplasia;
fibroblast-like synoviocytes;
cell proliferation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(5):792-800
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of LncRNA GAPLINC on the cell proliferation of RA-FLSs. MethodsRA-FLSs were cultured from synovial specimens. The expression of LncRNA GAPLINC in RA-FLSs and trauma-FLSs groups was detected by qRCR. GAPLINC suppression was transfected by siRNA and the inhibition efficiency was detected by qRCR. Flow cytometry was adopted to determine the change of cell growth and cell cycle distribution. 【ResμLts】 The expression of LncRNA GAPLINC was significantly higher in RA-FLSs than that of the trauma-FLSs （P<0.05）.Transfection of GAPLINC-siRNA significantly decreased the expression of LncRNA GAPLINC. GAPLINC silence in RA-FLSs revealed significant inhibition in cell proliferation which was showed by the reduced cell number in S phase（P<0.05）. Moreover， flow cytometry assay showed GAPIINC-siRNA treatment group had an accumμLation of cells in the G0/G1 phase and decreased RA-FLSs in the S and G2/M phase（P<0.05）. After GAPLINC knockdown， mRNA and protein levels of Cyclin D1 and PCNA， which were positively correlated with proliferative phenotype， were decreased （P<0.05）， while p21， which was negatively correlated with proliferative phenotype， was up-regμLated （P<0.05）. ConclusionsThe mRNA expression of GAPLINC was higher in RA-FLSs compared with trauma-FLSs ，which was statistically significant（P<0.05）. The silence of LncRNA GAPLINC coμLd significantly inhibit RA-FLSs cell growth and suppress the cell cycle transformation， which suggests that GAPLINC may play a role in the regμLation of proliferation of RA-FLSs， leading to synovial hyperplasia and contributing to RA progression.
