Isolation and morphological identification of endophytic fungi from leaves and stems of Aquilaria malaccensis Lamk. originating from Java Island, Indonesia

Abdul Salim; Tri Rini Nuringtyas

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Isolation and morphological identification of endophytic fungi from leaves and stems of Aquilaria malaccensis Lamk. originating from Java Island, Indonesia

Author: Abdul Salim ¹ ; Tri Rini Nuringtyas ^{2
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Author Information

1. Graduate Study Program, Faculty of Biology, Universitas Gadjah Mada, Jl Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia.
2. Tropical Biology Department, Faculty of Biology, Jl Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia. &
3. Research Center for Biotechnology, Universitas Gadjah Mada Jl. Teknika Utara, Kocoran, Yogyakarta, 55281, Indonesia.
Publication Type:Journal Article
Keywords: Aquilaria malaccensis; Endophytic fungi; Agarwood; Fungal identification
MeSH: Endophytes; Thymelaeaceae
From:Malaysian Journal of Microbiology 2023;19(1):55-62
CountryMalaysia
Language:English
Abstract: Aims:Endophytic fungi are the remarkable category of host-associated fungal community that invades the intercellular regions of host tissues, benefiting their host while obtaining an advantage. Fungal endophytes have lately attracted prominence as a source of active secondary metabolites. This investigation aimed to identify fungal endophytes that reside inside the leaves and stems of Aquilaria malaccensis.
Methodology and results:Healthy A. malaccensis stems and leaves samples were collected. Clean leaves and stems were cut to a size of 1 cm, followed by sterilization using 75% ethanol for 1 min, 3% sodium hypochlorite solution for 1 min, and finally, rinsing with sterile water 3 times for 1 min and drying with sterile paper. The sterile samples were put onto Potato Dextrose Agar (PDA) media containing chloramphenicol for 7-14 days until the mycelium grew for morphological identification under a light microscope. Five endophytic fungi were recovered from leaves, while nine endophytic fungi were obtained from stems. Using morphological approaches, nine of the endophytes had observed to produce conidia fungi, whereas the others did not. Neopestalotiopsis sp., Aspergillus sp., Arthrinium sp., Curvularia sp., Podospora sp., Mucor sp. and Verticillium sp. were identified as nine of the fourteen endophytes.
Conclusion, significance and impact of study:The number of endophytic fungi discovered in different organs varies. Not all endophytic fungi that grow can create sexual phages. Six genera of endophytic fungi were identified.
Full text:20.2023my0007.pdf