Expression of circ_0006692 in Non-small Cell Lung Cancer and Its Regulatory Mechanism on Proliferation and Metastasis of Lung Cancer Cells
10.3971/j.issn.1000-8578.2021.21.0383
- VernacularTitle:非小细胞肺癌组织中circ_0006692的表达及其对肺癌细胞增殖转移的调控机制
- Author:
Zeng CHEN
1
;
Jinrong LIAO
;
Changyan ZOU
;
Ying SU
;
Keyu LIN
;
Shanfeng JIN
;
Qianlan ZHENG
;
Xiandong LIN
Author Information
1. Laboratory of Radiation Oncology and Radiobiology, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou 350014, China
- Publication Type:Research Article
- Keywords:
circ_0006692;
Non-small cell lung cancer;
Proliferation;
Metastasis;
EMT
- From:
Cancer Research on Prevention and Treatment
2021;48(9):846-852
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of circ_0006692 in NSCLC and its relation with clinicopathological characteristics and related mechanism. Methods We collected 50 pairs of NSCLC tissues and adjacent tissues. The expression of circ_0006692 was detected by qRT-PCR and its relation with clinicopathological features was analyzed. We constructed lung cancer A549 cell line with circ_0006692 overexpression and knockdown. Cell proliferation, migration and invasion were detected by MTS, colony forming, wound healing and Transwell invasion assays. qRT-PCR and Western blot were used to detect the effect of circ_0006692 expression change on EMT-related gene expression. Results The expression of circ_0006692 in NSCLC was significantly higher than that in adjacent tissues (P < 0.05). The expression level of circ_0006692 was closely related to tumor size, TNM stage and pulmonary membrane invasion (P < 0.05). circ_0006692 knockdown inhibited cell proliferation, invasion and metastasis, while its overexpression promoted cell proliferation, invasion and metastasis. circ_0006692 knockdown inhibited the expression of EMT-related genes CDH2 and MMP7 in A549 cells and promoted the expression of CDH1. Conclusion The upregulated expression of circ_0006692 in NSCLC is closely related to tumor size, TNM stage and pulmonary membrane invasion. circ_0006692 expression could regulate the proliferation, invasion, metastasis and EMT progression of lung cancer A549 cells.
