PDA-mediated Mild Photothermal Therapy Combined with Autophagy Inhibitors Kill Breast Cancer Cells
10.3971/j.issn.1000-8578.2021.21.0142
- VernacularTitle:PDA介导的温和光热联合自噬抑制剂杀伤乳腺癌细胞
- Author:
Yawen LIU
1
;
Jiahui LU
;
Chen NI
;
Jie HUANG
;
Tianhao HUANG
;
Nan SHEN
;
Yulin DONG
;
Meilin SHI
;
Junfeng HU
Author Information
1. School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China
- Publication Type:Research Article
- Keywords:
Near-infrared laser;
Autophagy inhibition;
Mild photothermal therapy
- From:
Cancer Research on Prevention and Treatment
2021;48(7):659-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether inhibiting autophagy can enhance the sensitivity of photothermal treatment under mild photothermal conditions. Methods CQ@PLGA@PDA NPs were prepared by an improved double emulsification method and a PDA-based surface modification method. After basic characterization, CCK-8 method was used to detect the cytotoxicity of nanoparticles; the near-infrared laser irradiation nanoparticle solution was used to detect the heating effect; CCK-8 method and live-dead cell staining were used to detect the killing effect of tumor cells; Western blot was used to detect the expression of autophagy-related proteins. Results The CQ@PLGA@PDA NPs were successfully prepared, with a particle size of 253.10±2.39 nm, a zeta potential of -22.57±0.80 mV, uniform particle size and good dispersion. The temperature of nanoparticle solution increased to 45℃ after the near-infrared laser irradiation for 10 min. CQ@PLGA@PDA NPs had no obvious toxicity to cells. The survival rates of breast cancer cell MDA-MB-231 and mouse embryonic fibroblast NIH-3T3 cell were above 95%. The inhibition of autophagy under mild photothermal conditions could improve the sensitivity of photothermal therapy. Conclusion The prepared CQ@PLGA@PDA NPs have good photothermal performance and high biological safety; by inhibiting autophagy, they can effectively kill tumor cells under mild photothermal conditions(< 50℃).
