LncRNA WT1-AS Knockdown Promotes Invasion and Migration of Triple Negative Breast Cancer MDA-MB-231 Cells
10.3971/j.issn.1000-8578.2021.20.0744
- VernacularTitle:沉默LncRNA WT1-AS对三阴性乳腺癌细胞MDA-MB-231侵袭和迁移的影响
- Author:
Jiawu SHU
1
;
Xijun LI
;
Liqin ZHU
;
Yanyan WANG
Author Information
1. Department of Pharmacy, Yichang Central People's Hospital, Yichang 443003, China
- Publication Type:Research Article
- Keywords:
LncRNA WT1-AS;
Triple negative breast cancer cells;
Invasion;
Migration
- From:
Cancer Research on Prevention and Treatment
2021;48(3):244-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of LncRNA WT1-AS on the invasion and migration of triple-negative breast cancer MDA-MB-231 cells. Methods qRT-PCR was used to detect the expression of WT1-AS and the efficiency of gene silencing in MDA-MB-231 cells. Transwell invasion test and scratch test were used to detect the invasion and migration of MDA-MB-231 cells; Western blot was used to detect the expression of E-cadherin, N-cadherin, Vimentin and Snail in MDA-MB-231 cells. Results Compared with normal human mammary epithelial cells, the expression of WT1-AS in MDA-MB-231, MDA-MB-453 and MDA-MB-468 cells were significantly up-regulated. Knockdown of WT1-AS significantly reduced the invasion and migration abilities of MDA-MB-231 cells, the expression of E-cadherin was increased but N-cadherin, Vimentin and Snail expression were decreased. Conclusion WT1-AS could promote the invasion and migration of triple-negative breast cancer cells MDA-MB-231 via regulating epithelial-mesenchymal transition.
