Role and Possible Mechanism of LncRNA-MEG3 and KLF4 in Carcinogenesis and Progression of Nasopharyngeal Carcinoma
10.3971/j.issn.1000-8578.2021.20.0790
- VernacularTitle:LncRNA-MEG3和KLF4在鼻咽癌发生发展中的作用及可能机制
- Author:
Jiayu NING
1
;
Weiijing BAO
;
Sujuan ZHOU
;
Rifu WEI
;
Zhongshou ZHU
Author Information
1. Department of Pathology, Ningde Municipal Hospital, Ningde 352100, China
- Publication Type:Research Article
- Keywords:
Nasopharyngeal carcinoma cells;
Maternally expressed gene 3;
miR-543;
KLF4
- From:
Cancer Research on Prevention and Treatment
2021;48(3):234-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of LncRNAs-MEG3 in the carcinogenesis and progression of nasopharyngeal carcinoma (NPC) and the possible molecular mechanism. Methods qRT-PCR was used to detect the content of MEG3 and miR-543 in NPC cells. Luciferase reporter method was used to study the relation between MEG3 and miR-543, and the changes of cell proliferation and apoptosis induced by MEG3 or KLF4 were analyzed. Western blot was used to detect the expression of KLF4, Bcl-2 and Bax proteins. Results Compared with the control group, the expression of miR-543 in NPC cell line was significantly increased (P < 0.05), while the expression of MEG3 was decreased (P < 0.05). Luciferase report and Western blot showed that MEG3 could regulate the expression of KLF4 by adsorbing miR-543 to inhibit cell proliferation, promote cell apoptosis and affect the expression levels of Bcl-2 and Bax proteins. Conclusion LncRNA-MEG3 could regulate the expression of KLF4 by adsorbing miR-543 and then plays a role in inhibiting the occurrence and development of NPC. It may be a new biomarker for NPC targeted therapy.
