Application of New Single-cell Analysis Technology——Cytometry by Time-of-flight in Treatment of Leukemia
10.3971/j.issn.1000-8578.2021.20.0662
- VernacularTitle:新型单细胞分析技术——质谱流式细胞术在白血病治疗中的应用
- Author:
Hongjuan TIAN
1
;
Renle ZHANG
;
Yanhong QIAO
;
Dengxia ZHANG
;
Bei LIU
Author Information
1. The First Clinical Medical College of Lanzhou University, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
Cytometry by time-of-flight;
Leukemia;
Therapeutic applications
- From:
Cancer Research on Prevention and Treatment
2021;48(2):209-213
- CountryChina
- Language:Chinese
-
Abstract:
Cytometry by time-of-flight (CyTOF) is an innovative single-cell analysis technique combining with mass spectrometry principles and flow cytometry. In recent years, CyTOF has been widely used in the study of leukemia treatment for its advantages of multi-parameter, high-throughput, compensation without calculation and diversified data analysis. It can accurately determine intracellular element contents and analyze the changes of unique phenotype and complex signaling pathways of leukemia cells at the single-cell level, to study the mechanism of action of anti-leukemia drugs, discover the potential therapeutic targets of leukemia, assess the therapeutic response and deeply understand the mechanism of leukemia relapse and drug resistance. This article reviews the research progress of CyTOF principle, characteristics, advantages and its application in leukemia treatment.
