Prevalence of CYP2C19 681G>A and 636G>A gene polymorphisms in Javanese farmers exposed to Chlorpyrifos
https://doi.org/10.47895/amp.v56i1.4498
- Author:
Liem Jen Fuk
1
;
Muchtaruddin Mansyur
2
;
Safarina G. Malik
3
;
Dwi A. Suryandari
4
;
Dewi S. Soemarko
2
;
Imam Subekti
5
;
Franciscus D. Suyatna
6
;
Aria Kekalih
2
;
Bertha Pangaribuan
7
Author Information
1. Department of Occupational Health and Safety, Faculty of Medicine and Health Science, Universitas Kristen Krida Wacana
2. Community Medicine Department, Faculty of Medicine Universitas Indonesia
3. Eijkman Institute for Molecular Biology, National Research and Innovation Agency
4. Department of Biology, Faculty of Medicine Universitas Indonesia
5. Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia and Dr. Cipto Mangunkusumo General Hospital
6. Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia
7. Prodia Occupational Health Institute International
- Publication Type:Journal Article
- Keywords:
Susceptibility biomarker
- MeSH:
Cytochrome P-450 CYP2C19;
Pesticides
- From:
Acta Medica Philippina
2022;56(1):7-11
- CountryPhilippines
- Language:English
-
Abstract:
Objective:The objective of our study was to determine the genotype frequencies of CYP2C19*2 and *3 gene polymorphisms among Javanese farmers exposed to chlorpyrifos (CPF) in Central Java, Indonesia.
Methods:This cross-sectional study was conducted from July to October 2020 in Central Java, Indonesia, involving 151 vegetable farmers aged 18-65 years who used CPF for at least one year. CYP2C19*2 and *3 gene polymorphisms were analyzed using PCR-RFLP. Direct calculations were applied to calculate allele and genotype frequencies. The difference in genotype frequencies among the sex and cumulative exposure level (CEL) group was performed using the Chi-square test. In contrast, the proportion difference of allele frequencies was analyzed using Z-test.
Results:The frequency of CYP2C19 genotypes *1/*1, *1/*3, *1/*2, and *2/*2 were 64 %, 7 %, 23 % and 6 %. We observed no significant difference in the genotype distribution according to sex group and CEL group.
Conclusion:In summary, the prevalence of toxicologically relevant CYP2C19 polymorphisms was determined in the Javanese agricultural population. The CYP2C19 genotype may be helpful as an essential biomarker of genetic susceptibility towards CPF exposure. Nevertheless, further studies to confirm the role of CYP2C19 in this context are still needed.
- Full text:4498-Article Text-54178-1-10-20220131.pdf
