Preparation and Performance of Three-Dimensional Silk FibroinScaffolds with Different Matrix Stiffness
10.16156/j.1004-7220.2023.01.21
- VernacularTitle:不同基质刚度三维丝素支架制备及其性能研究
- Author:
Shiyun YIN
1
;
Yaxin DENG
1
;
Guobao CHEN
1
Author Information
1. School of Pharmacy and Bioengineering, Chongqing University of Technology
- Publication Type:Journal Article
- Keywords:
silk fibroin (SF);
sodium alginate (SA);
collagen (COL);
matrix stiffness;
bone tissue engineering
- From:
Journal of Medical Biomechanics
2023;38(1):E142-E148
- CountryChina
- Language:Chinese
-
Abstract:
Objective After hydrogen bonding between collagen ( COL) and silk fibroin ( SF ) at different concentrations, a composite scaffold with adjustable stiffness was prepared by combining with gel system, and its physical and chemical properties were characterized. Methods SF with different qualities was dissolved in sodium alginate (SA) solution, then COL solution at different concentration and calcium carbonate ( CaCO3 ) powder were added. The hydrogels of SC1, SC2, and SC3 groups were obtained by taking out the mixed solution and adding some gluconic acid lactone ( GDL) powder, and different SF scaffolds were obtained after freeze drying. Results The SF scaffolds with adjustable stiffness were successfully prepared. The compression moduli of SC1, SC2, and SC3 groups were (17. 31±2. 73), (24. 12±1. 81), (32. 54±1. 81) kPa, respectively. The innerstructure of the scaffolds was observed. From SC1 group to SC3 group, pores of the scaffolds were smaller and fewer, and hydrophilicity of the materials become better and better. Conclusions Three-dimensional ( 3D) porous scaffolds with different matrix stiffness can be prepared by changing the concentration of SF and COL solution. The concentration of SF and COL is proportional to the compression modulus, water absorption, water retention and swelling rate of SF scaffolds, while inversely proportional to porosity. The findings of this study are expected to provide theoretical guidance for construction of scaffolds with appropriate matrix stiffness for inducing osteogenic differentiation of mesenchymal stem cells
