In Vitro Antioxidant Properties and Methylglyoxal (MGO) Scavenging Effects of Centella asiatica Leaves in Water Extract
https://doi.org/10.47836/mjmhs.18.6.25
- Author:
Xiu Qing Chong
1
;
Li Ann Saw
1
;
Jia Ying Puah
1
;
Kirthani AP Anamalay
1
;
Patrick Nwabueze Okechukwu
2
;
Hor Kuan Chan
1
Author Information
1. Department of Food Science with Nutrition, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, (Taman Connaught) Cheras, 56000 Kuala Lumpur, Malaysia
2. Department of Biotechnology, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, (Taman Connaught) Cheras, 56000, Kuala Lumpur, Malaysia
- Publication Type:Journal Article
- Keywords:
C. asiatica L., AGE, MGO, antioxidant
- From:Malaysian Journal of Medicine and Health Sciences
2022;18(No.6):183-192
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Advanced glycation end product (AGE) is formed by the non-enzymatic glycation, leading to free
radicals’ production and high oxidative stress. Accumulation of AGEs is associated with chronic diseases which
are correlated with diabetes mellitus. Centella asiatica is a traditional medicinal herb used in Asia with numerous
pharmacological effects such as antioxidant, wound healing, neuroprotective, and gastrointestinal treatment. This research was aim to evaluate the antioxidant, antiglycation, and phytochemical analysis of C. asiatica leaves (L.) water
extract. Methods: The antioxidant activity of the plant extract was evaluated through 2,2-diphenyl-1-picrylhydrazyl
(DPPH), hydroxyl (OH) radicals, nitric oxide (NO), and chelating capacity assays. Total phenolic content (TPC) was
performed to determine the presence of bioactive compounds, such as saponins, which have been extensively reported in previous studies. Butylated hydroxytoluene (BHT) and ethylenediaminetetraacetic acid (EDTA) were used
as positive control in the antioxidant assays, while quercetin was used in the antiglycation assays. Antiglycation
effects of C. asiatica L. were evaluated through Bovine serum albumin (BSA)-MGO, BSA-glucose, and MGO scavenging assay. The data was expressed in terms of IC50. Result: The plant extract possessed significantly (p < 0.001) lower
IC50 value compared to the BHT in DPPH and NO radical scavenging assay. It also showed stronger antiglycative
effect with lower IC50 value compared to the quercetin in BSA-glucose and BSA-MGO assay. The TPC of C. asiatica
L. was 3.20 mg/GAE/g at 100µg/ml. Conclusion: The antiglycation effects of C. asiaitca L. include the reduction of
Amadori products formation, trapping α-dicarbonyl intermediates, and free radical scavenging. It is suggested that
the antiglycation effects of C. asiatica L. maybe attributed by the presence of saponins and other phytochemicals.
- Full text:11.2022my1375.pdf
