Advance on detection method and application based on bioluminescence of luciferase
10.11665/j.issn.1000-5048.2023021401
- VernacularTitle:基于荧光素酶生物发光检测方法的研究及其应用进展
- Author:
Lin LIU
1
;
Tingting HU
;
Mengling WANG
;
Yao NIE
;
Weijie ZHANG
;
Chen WANG
;
Bingjie ZOU
;
Qinxin SONG
;
Guohua ZHOU
Author Information
1. 中国药科大学药物质量与安全预警教育部重点实验室
- Publication Type:Journal Article
- Keywords:
bioluminescence reaction;
luciferin;
luciferase;
detection method;
progress
- From:
Journal of China Pharmaceutical University
2023;54(4):389-398
- CountryChina
- Language:Chinese
-
Abstract:
Bioluminescence is a widespread phenomenon in nature, and luminescent organisms can be found both on land and in the ocean. Among them, luciferase based bioluminescence systems have been widely studied, inspiring the exploration of genetic and epigenetic aspects and the development of a series of related assays for in vivo and in vitro studies. This paper summarizes the recent developments of luciferase based bioluminescence assays in terms of bioluminescence systems, types of luciferases, and the development and application of luciferase bioluminescence assays.
