Interleukin-27 Disrupts the Crosstalk of Apoptotic Activities between 4T1 Breast Cancer Cells and M2 Macrophages
https://doi.org/10.47836/mjmhs.18.6.18
- Author:
Nurliyana Mohd Yusof
1
;
Natasha Nurafiqah Mohamed Noor Fuadi
1
;
Muhajir Hamid
2
;
Noorjahan Banu Mohamed Alitheen
1
;
Nursyuhaida Mohd Hanafi
3
;
Nik Mohd Afizan Nik Abd Rahman
1
,
4
Author Information
1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia&
2. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3. Agro-Biotechnology Institute Malaysia, National Institutes of Biotechnology Malaysia (NIBM), C/O Mardi Headquaters, Serdang, Malaysia
4. Institute of Tropical Forest and Forestry Products, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Interleukin-27 (IL-27);
pcDNA3.4-IL27;
apoptosis;
M2 macrophages
- From:Malaysian Journal of Medicine and Health Sciences
2022;18(No.6):125-133
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Cytokine immunotherapy such as Interleukin-27 (IL-27) has been foreseen as a promising alternative anti-cancer treatment. Thus, this study aimed to investigate whether IL-27 gene therapy regulates crosstalk between breast cancer cells and macrophages in the sense of pro-apoptotic activities. Methods: This study has led to the development of recombinant pcDNA3.4-IL27. The recombinant pcDNA3.4-IL27 was transfected into 4T1 murine mammary carcinoma cells alone and co-culture of 4T1 with M2 macrophages. The successful expression of IL-27 in the cells were determine through the immunofluorescence staining and detection of CD206, M2 macrophages marker. Apoptotic effects of pcDNA3.4-IL27 were assessed through MTT assay, Annexin V flow cytometer analysis, and AO/PI dual staining. Results: Our findings shows that pcDNA3.4-IL27 has the ability to induce apoptosis in both of the cell group and performs better in the co-culture of 4T1 with M2 macrophages compared to 4T1 cells alone. PcDNA3.4-IL27 induced apoptosis through the altered cell morphology and reduction in the number of viable cells. Conclusion: These data demonstrate that pcDNA3.4-IL27 has the ability to induce apoptosis in both 4T1 cell alone and co-cultured 4T1 with M2 macrophages. Thus, could serve as a potential anti cancer candidate against breast cancer.
- Full text:11.2022my1368.pdf
