Effects of Yupingfeng granules on tumor metastasis of Lewis tumor-bearing mice by adjusting the EMT
- VernacularTitle:玉屏风颗粒通过调节EMT对Lewis荷瘤小鼠肿瘤转移的影响
- Author:
Yingxue LIN
1
;
Haipeng CUI
2
;
Lingdi WANG
1
;
Changshuo YANG
3
;
Pengwei ZHUANG
3
Author Information
1. Dept. of Pharmacy，the Affiliated Hospital of Chengde Medical University，Hebei Chengde 067000，China
2. Teaching and Research Section of Pathophysiology，Chengde Medical University，Hebei Chengde 067000，China
3. School of Chinese Materia Medica，Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China
- Publication Type:Journal Article
- Keywords:
Yupingfeng granules;
epithelial-mesenchymal
- From:
China Pharmacy
2023;34(17):2090-2094
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effects of Yupingfeng granules on the improvement of epithelial barrier function and the inhibition of tumor metastasis by regulating epithelial-mesenchymal transition （EMT）. METHODS Thirty C57BL/6 mice were randomly divided into the normal group （distilled water），model group （distilled water） and Yupingfeng granule group （40 g/kg）， with 10 mice in each group. The model group and Yupingfeng granule group were inoculated with Lewis lung cancer cells subcutaneously in the right armpit to induce the spontaneous lung metastasis model. After modeling，each group was given water/ relevant medicine intragastrically，once a day，for 15 consecutive days. The effects of Yupingfeng granules on tumor metastasis were investigated by observing or determining the pathomorphology of lung tissue，metastatic lesion count on the surface of the lung， tumor metastatic lesion and the expression of carcinoembryonic antigens. qRT-PCR and Western blot assay were used to detect the mRNA and protein expressions of β-catenin，E-cadherin and vimentin in the lung tissue of mice. RESULTS Compared with the model group，the total number of pulmonary metastases on the surface was decreased significantly in Yupingfeng granule group （P＜ 0.05），the general morphology of lung tissue was recovered，and the expression of carcinoembryonic antigen in lung tissue was significantly decreased （P＜0.05）. mRNA and protein expressions of E-cadherin in lung tissue were significantly increased （P＜ 0.05），while mRNA and protein expressions of vimentin and β-catenin were significantly decreased （P＜0.05）. CONCLUSIONS Yupingfeng granules can inhibit EMT by regulating the expression of β-catenin，thus improving epithelial barrier function，and inhibiting the ability of tumor cells to invade and metastasize.
