Content determination of 6 components in Jinlian qingre granules by QAMS method based on a variety of internal reference substances
- VernacularTitle:基于多种内参物的一测多评法测定金莲清热颗粒中6种成分含量
- Author:
Min HE
1
;
Shan MAO
1
;
Lin LI
2
;
Haifeng NI
2
;
Qingyu DU
1
;
Yongjie YU
1
,
3
,
4
;
Xia ZHANG
1
,
3
Author Information
1. School of Pharmacy，Ningxia Medical University，Yinchuan 750004，China
2. Ningxia Qiyuan Pharmaceutical Co. Ltd.，Yinchuan 750011，China
3. Ningxia Key Laboratory of Drug Development and Generic Drug Research，Yinchuan 750004，China
4. Key Laboratory of Ningxia Minority Medicine Modernization，Ministry of Education，Yinchuan 750004，China
- Publication Type:Journal Article
- Keywords:
Jinlian qingre granules;
quantitative analysis
- From:
China Pharmacy
2023;34(17):2069-2073
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a quantitative analysis of multi-components by single marker （QAMS） method based on a variety of internal reference substances for the content determination of 6 components in Jinlian qingre granules， such as mangiferin， 2″-O-β-L-galactopyranosylorientin， orientin， veratric acid， vitexin， harpagoside. METHODS The determination was performed on Agilent Eclipse Plus C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （gradient elution） at the flow rate of 1 mL/min. The column temperature was 30 ℃， and the detection wavelength was set at 270 nm. Taking orientin， vitexin and 2″-O-β-L-galactopyranosylorientin as internal references， the relative correction factors （RCF） of the other 5 components to be determined and internal substances were determined by QAMS. The contents of 6 components in 21 batches of Jinlian qingre granules were calculated and then compared with the results of the external standard method. RESULTS The contents of mangiferin， 2″-O-β-L-galactopyranosylorientin， orientin， veratric acid， vitexin and harpagoside in 21 batches of samples were determined by QAMS in the range of 0.234-0.516， 1.804-2.270， 2.143-2.606， 0.190-0.223， 0.594-0.782， 0.080-0.152 mg/g； the contents of them determined by external standard method were 0.235-0.523， 1.798-2.265， 2.137-2.599， 0.190-0.224， 0.597-0.786， 0.077-0.151 mg/g， respectively. The percentage difference between the results measured by the two methods should not exceed 4.00%. CONCLUSIONS QAMS has been constructed for the simultaneous determination of 6 components in Jinlian qingre granules based on a variety of internal reference substances. The results obtained by this method are not significantly different from those obtained by the external standard method， and can be used for the quality control of Jinlian qingre granules.
