Evaluation of Anti-Hyperlipidaemic Activity of a Mixture of  Zinger officinale, Allium sativum, Citrus Lemon, Honey, and  Malus domestica Vinegar (ZACAH) Extracts in Rats Fed with  High Cholesterol Diet

Kokila Vani Perumal; Hasnah Bahari; Norshafarina Shari; Kasturi Kanniappan; Khairul Kamilah Abdul Kadir; Zunoliza Abdullah; Mohd Radzi Ahmad; Ibrahim Kalle Kwaifa; Siti Hadizah Jumidil; Azrina Zainal Abidin; Sabariah Md Noor; Yong Yoke Keong; Santhra Segaran Balan

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Evaluation of Anti-Hyperlipidaemic Activity of a Mixture of Zinger officinale, Allium sativum, Citrus Lemon, Honey, and Malus domestica Vinegar (ZACAH) Extracts in Rats Fed with High Cholesterol Diet

Author: Kokila Vani Perumal ¹ ; Hasnah Bahari ¹ ; NorShafarina Shari ² ; Kasturi Kanniappan ² ; Khairul Kamilah Abdul Kadir ³ ; Zunoliza Abdullah ³ ; Mohd Radzi Ahmad ³ ; Ibrahim Kalle Kwaifa ^{1
,

4} ; Siti Hadizah Jumidil ¹ ; Azrina Zainal Abidin ^{1
,

2} ; Sabariah Md Noor ¹ ; Yong Yoke Keong ¹ ; Santhra Segaran Balan ^{1
,

2}
Author Information

1. Faculty Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia&
2. Faculty of Health and Life Sciences, 40100 Shah Alam, Selangor, Malaysia
3. Institut Penyelidikan Perhutanan Malaysia (FRIM), 52109 Kepong, Selangor
4. School of Medical Laboratory Sciences, College of Health Sciences, Usmanu Danfodiyo University (UDU), Sokoto, NorthWestern 2346, Nige
Publication Type:Journal Article
Keywords: ZACAH extracts, Hyperlipidaemia, High cholesterol diet
From:Malaysian Journal of Medicine and Health Sciences 2022;18(No.5):55-63
CountryMalaysia
Language:English
Abstract: Introduction: The incidence and complications of hyperlipidemia associated co-morbidities and mortalities are grossly increasing globally. Intervention with natural products preparation has been identified as an alternative for the management of hyperlipidaemia and its related cardiovascular complications. This study investigated the anti-hyperlipidaemic activities of a mixture of Zinger officinale, Allium sativum, Citrus lemon, honey, and Malus domestica vinegar (ZACAH) extracts in in Sprague Dawley (SD) rats fed with high cholesterol diet. Methods: Thirty-six male SD rats were randomly distributed into 6 groups, including normal chow diet-fed (NC), high cholesterol diet (HCD), HCD+ Simvastatin (standard drug) while the remaining three groups were fed with HCD + ZACAH extracts at different doses (1, 3 and 5mg/kg body weight) for 18 weeks. Simvastatin at 10 mg/kg of bodyweight was used as control. High-performance liquid chromatography (HPLC) was used to determine phenolic compounds present in ZACAH extracts, elastase inhibitory assay was determined using spectrometric with a substrate (N-Succ-(Ala)3-pnitroanilide (SANA) while 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed based by the method described by Blois, 1958. Results: In vitro; ZACAH extracts had oxygen radical absorbance capacity (ORAC) value of 2000 µmol TE/100 mL, total phenolic content (TPC) of 7537 ± 54.5%, DPPH free radical scavenging activity of 27.34 ± 2.71%, elastase inhibitory assay of 29.29 ± 1.65% and lipoxygenase inhibitory assay of 98.58 ± 1.42%. In vivo, ZACAH extracts showed decreased bodyweight, adipose tissue, improved lipid profiles and hepatic biochemical enzymes. Conclusion: These results suggested that ZACAH extracts supplementation improved hyperlipidaemia in SD rats and might be a promising adjuvant for the treatment of hyperlipidaemia.
Full text:11.2022my1328.pdf