Upregulation of ppET-1/ETBR/eNOS mRNA Expression After Calcitriol Treatment in Chronic Kidney Diseases Model in Rats
https://doi.org/10.47836/mjmhs.18.5.4
- Author:
Anak Agung Ngurah Nata Baskara
1
,
2
;
Nur Arfian
1
;
Wiwit Ananda Wahyu Setyaningsih
1
;
Muhammad Mansyur Romi
1
,
3
;
Dwi Cahyani Ratna Sari
1
Author Information
1. Department of Anatomi, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, 55281, Special Region of Yogyakarta, Indonesia&
2. Master Program in Biomedical Sciences, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta , 55281, Special Region of Yogyakarta
3. Faculty of Medicine, Universitas Muhammadiyah Purwokerto, Purwokerto, 53182, Central Java, Indonesia
- Publication Type:Journal Article
- Keywords:
Chronic kidney disease, Calcitriol, Endothelin-1, eNOS, Endothelin receptors
- From:Malaysian Journal of Medicine and Health Sciences
2022;18(No.5):23-29
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Myofibroblast formation in the interstitial area is the hallmark of chronic kidney disease (CKD). Endothelin signalling has been known to play role in physiology and pathophysiology in the kidney. Vitamin D has a
reno-protective effect through inhibiting inflammation and fibrosis. However, the interaction between vitamin D and
endothelin signalling in the CKD model has not been elucidated yet. Therefore, we aimed to check the difference
impact of endothelin (ET) receptor in CKD. Methods: Sprague Dawley rats (3-months-old, 150-250grams) underwent
5/6 subtotal nephrectomy (SN) to induce CKD. Then, it was divided into 4 groups (each contains 6 rats): sham operation (SO), 5/6 subtotal nephrectomy (SN), calcitriol groups (0.01µg/100grBW/day (SN-D1), and 0.05µg/100grBW/day
(SN-D2). Calcitriol was administered for 14 days after the surgery. The Sham Operation (SO) group was injected with
NaCl. At the specified date, the rats were sacrificed and the kidneys were harvested. Fibrosis was quantified based
on Sirius Red staining. Immunostaining was done for localizing fibroblast (PDGFRβ). The mRNA expressions of prepro-ET-1, endothelin receptor A (ETAR), endothelin receptor B (ETBR), and endothelial nitrite oxide synthase (eNOS)
were quantified using reverse-transcriptase PCR (RT-PCR). Results: The CKD promotes an elevation of prepro-ET-1,
ETBR, and eNOS, and reduction of ETAR (p<0.05) mRNA expression compared to the SO group. Administration of
calcitriol (SN-D1 and SN-D2) showed the vice versa effects. However, only SN-D2 group consistently showed statistically significant differences whenever compared to either SO or SN groups. Conclusion: Calcitriol might attenuate
interstitial fibrosis in CKD model via ET-1/eNOS signalling.
- Full text:11.2022my1323.pdf
