Co-expression of circRNA in oral leukoplakia and oral lichen planus
10.12016/j.issn.2096-1456.2023.11.003
- Author:
YANG Jingwen
1
,
2
;
SONG Yuhan
1
,
2
;
XU siming
1
,
2
;
GE Shuyun
1
,
2
;
ZHOU Haiwen
1
,
2
Author Information
1. Department of Oral Mucosal Diseases, Shanghai Ninth People'
2. s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University National center for Stomatoloy, National Clinical Research Center for oral diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology
- Publication Type:Journal Article
- Keywords:
circRNA / circHLA-C / oral leukoplakia / oral lichen planus / oral potentially malignant disorders / malignant transformation / biomarkers / high-throughput sequencing
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2023;31(11):774-781
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To find any differentially expressed circRNAs in oral leukoplakia (OLK) and oral lichen planus (OLP), to investigate the possible role of circRNAs in the pathogenesis of these two diseases.
Methods: This study obtained hospital ethical approval. High-throughput sequencing was used to detect differentially expressed circRNAs in OLK, OLP, oral squamous cell carcinoma and normal oral mucosal tissues. CircRNAs were verified by qRT-PCR, enzyme tolerance assays and Sanger sequencing. GO functional analysis and KEGG pathway analysis were performed to predict the functions of circRNAs in OLP. TargetScan and miRanda were applied to predict targeted miRNAs and mRNAs of circRNAs, and ceRNA networks were mapped.
Results:A total of 49 circRNAs were differentially expressed in OLK and OLP together, including 30 upregulated and 19 downregulated circRNAs. The five circRNAs confirmed with RT-qPCR, including circHLA-C, circRNF13, circTTN, circSEPN2 and circALDH3A2, were all abnormally expressed in OLK and OLP, among which circHLA-C was a key circRNA with trans splice sites, which was validated by expanding the sample size. ROC curve analysis showed that the area under the circHLA-C curve for predicting OLK was 0.955, and the area under the circHLA-C curve for predicting OLP was 0.988. GO functional analysis showed enrichment of many biological processes related to the immune process. The KEGG pathway with the highest enrichment score was "Natural killer cell mediated cytotoxicity". HLA-C was significantly enriched in these processes/pathways. CeRNA network analysis showed that circHLA-C interacted with a variety of miRNAs, such as hsa-miR-26a-5p, hsa-miR-129-5p, and hsa-miR-29a-3p.
Conclusion:Many circRNAs were differentially expressed in both OLK and OLP, circHLA-C being the most elevated. CircHLA-C is valuable for the early diagnosis of OLK and OLP and may serve as a potential biomarker for the diagnosis and prognosis of OLK and OLP.
- Full text:环状RNA在口腔白斑和口腔扁平苔藓中的共同表达.pdf
