Role of gut microbiota in perioperative neurocognitive disorders after cardiopulmonary bypass surgery in rats with humanized gut flora.
10.12122/j.issn.1673-4254.2023.06.11
- Author:
Jianing FAN
1
;
Yingjie SUN
2
;
Bing LIANG
2
;
Xiaoyan ZHANG
2
;
Cheng XIAO
3
;
Zeqing HUANG
1
Author Information
1. Department of Anesthesiology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China.
2. Department of Anesthesiology, General Hospital of Northern Theater Command, Shenyang 110016, China.
3. Health Service Team, No.96852 Troop of PLA, Shenyang 110124, China.
- Publication Type:Journal Article
- Keywords:
cardiopulmonary bypass;
gut-brain axis;
macrogenomics;
perioperative neurocognitive disorders
- MeSH:
Male;
Animals;
Rats;
Rats, Sprague-Dawley;
Cardiopulmonary Bypass;
Gastrointestinal Microbiome;
Interleukin-6;
Tumor Necrosis Factor-alpha;
Neurocognitive Disorders
- From:
Journal of Southern Medical University
2023;43(6):964-969
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate whether gut microbiota disturbance after cardiopulmonary bypass (CPB) contributes to the development of perioperative neurocognitive disorders (PND).
METHODS:Fecal samples were collected from healthy individuals and patients with PND after CPB to prepare suspensions of fecal bacteria, which were transplanted into the colorectum of two groups of pseudo-germ-free adult male SD rats (group NP and group P, respectively), with the rats without transplantation as the control group (n=10). The feces of the rats were collected for macrogenomic sequencing analysis, and serum levels of IL-1β, IL-6 and TNF-α were measured with ELISA. The expression levels of GFAP and p-Tau protein in the hippocampus of the rats were detected using Western blotting, and the cognitive function changes of the rats were assessed with Morris water maze test.
RESULTS:In all the 3 groups, macrogenomic sequencing analysis showed clustering and clear partitions of the gut microbiota after the transplantation. The relative abundances of Klebsiella in the control group (P < 0.005), Akkermansia in group P (P < 0.005) and Bacteroides in group NP (P < 0.005) were significantly increased after the transplantation. Compared with those in the control group, the rats in group NP and group P showed significantly decreased serum levels of IL-1β, IL-6 and TNF-α and lowered expression levels of GFAP and p-Tau proteins (all P < 0.05). Escape platform crossings and swimming duration in the interest quadrant increased significantly in group NP (P < 0.05), but the increase was not statistically significant in group N. Compared with those in group P, the rats in group NP had significantly lower serum levels of IL-1β, IL-6 and TNF-α and protein expressions of GFAP and p-Tau (all P < 0.05) with better performance in water maze test (P < 0.05).
CONCLUSION:In patients receiving CPB, disturbances in gut mirobiota contributes to the development of PND possibly in relation with inflammatory response.
