Silencing RAB27a inhibits proliferation, invasion and adhesion of triple-negative breast cancer cells.
10.12122/j.issn.1673-4254.2023.04.08
- Author:
Li WANG
1
;
Zhirui YAN
1
;
Yaoxiong XIA
1
Author Information
1. Department of Radiation Oncology, Yunnan Provincial Cancer Hospital (Third Affiliated Hospital of Kunming Medical University), Kunming 650118, China.
- Publication Type:Journal Article
- Keywords:
RAB27a;
RAB27b;
cell biological behaviors;
exosomes;
triple-negative breast cancer
- MeSH:
Humans;
rab GTP-Binding Proteins/metabolism*;
Triple Negative Breast Neoplasms;
Cell Line, Tumor;
rab27 GTP-Binding Proteins/metabolism*;
RNA, Small Interfering/genetics*;
Cell Proliferation/genetics*;
Gene Expression Regulation, Neoplastic
- From:
Journal of Southern Medical University
2023;43(4):560-567
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of inhibition of RAB27 protein family, which plays a pivotal role in exosome secretion, on biological behaviors of triple-negative breast cancer cells.
METHODS:Quantitative real-time PCR and Western blotting were used to examine the expressions of RAB27 family and exosome secretion in 3 triple-negative breast cancer cell lines (MDA-MB-231, MDA-MB-468, and Hs578T) and a normal breast epithelial cell line (MCF10A). The effect of small interfering RNA (siRNA)-mediated silencing of RAB27a and RAB27b on exosome secretion in the 3 breast cancer cell lines was detected using Western blotting, and the changes in cell proliferation, invasion and adhesion were evaluated.
RESULTS:Compared with normal breast epithelial cells, the 3 triple-negative breast cancer cell lines exhibited more active exosome secretion (P < 0.001) and showed significantly higher expressions of RAB27a and RAB27b at both the mRNA and protein levels (P < 0.01). Silencing of RAB27a in the breast cancer cells significantly down-regulated exosome secretion (P < 0.001), while silencing of RAB27b did not significantly affect exosome secretion. The 3 breast cancer cell lines with RAB27a silencing-induced down-regulation of exosome secretion showed obvious inhibition of proliferation, invasion and adhesion (P < 0.01) as compared with the cell lines with RAB27b silencing.
CONCLUSION:RAB27a plays central role in the exosome secretion in triple-negative breast cancer cells, and inhibiting RAB27a can inhibit the proliferation, invasion and adhesion of the cells.
