Effects of Celecoxib and Nordihydroguaiaretic Acid on Puromycin Aminonucleoside-Induced Nephrosis in the Rat.
10.3346/jkms.2009.24.S1.S183
- Author:
Dong Won LEE
1
;
Ihm Soo KWAK
;
Soo Bong LEE
;
Sang Heon SONG
;
Eun Young SEONG
;
Hyun Chul CHUNG
;
Byeong Yun YANG
;
Min Young LEE
;
Mee Young SOL
Author Information
1. Department of Internal Medicine, Pusan National University School of Medicine, Busan, Korea. iskwak@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Puromycin Aminonucleoside;
Proteinuria;
Celecoxib;
Nordihydroguaiaretic Acid
- MeSH:
Animals;
Anti-Inflammatory Agents, Non-Steroidal/pharmacology;
Body Weight;
Creatinine/blood;
Cyclooxygenase Inhibitors/pharmacology;
Male;
Microscopy, Electron;
Nephrosis/*chemically induced/drug therapy;
Nordihydroguaiaretic Acid/*pharmacology;
Podocytes/metabolism;
Puromycin Aminonucleoside/pharmacology/*toxicity;
Pyrazoles/*pharmacology;
Rats;
Rats, Sprague-Dawley;
Sulfonamides/*pharmacology;
Time Factors
- From:Journal of Korean Medical Science
2009;24(Suppl 1):S183-S188
- CountryRepublic of Korea
- Language:English
-
Abstract:
The selective cyclooxygenase-2 (COX-2) and 5-lipoxygenase (LOX) inhibitors might inhibit prostaglandin synthesis and reduce proteinuria. The present study was designed to investigate the anti-proteinuric effects of nordihydroguaiaretic acid (NDGA) as compared with celecoxib in puromycin aminonucleoside (PAN) nephrosis rats. Fifty five male Sprague-Dawley rats were divided into 4 groups; A, normal control; B, PAN group; C, PAN+COX-2 inhibitor (celecoxib) group; and D, PAN+5-LOX inhibitor (NDGA) group. After induction of PAN nephrosis through repeated injections of PAN (7.5 and 15 mg/100 g body weight), rats were treated with celecoxib, NDGA, or vehicle for 2 weeks. Twenty four hour urine protein excretions were significantly lower in PAN+celecoxib and PAN+NDGA groups than in PAN group. Serum creatinine (SCr) concentrations and 24 hr urine creatinine clearances (CCr) were not significantly different in the four groups. Electron microscopy showed that podocyte morphology was changed after the induction of PAN nephrosis and was recovered after celecoxib or NDGA administration. Celecoxib significantly recovered the expressions of nephrin, CD2AP, COX-2, and TGF-beta. NDGA also recovered TGF-betaexpression, but did not alter the expressions of nephrin, CD2AP and COX-2. The present study suggested that celecoxib and NDGA might effectively reduce proteinuria in nephrotic syndrome without impairing renal function.
