The Protective Effects of Green Tea Extract against L-arginine Toxicity to Cultured Human Mesangial Cells.
10.3346/jkms.2009.24.S1.S204
- Author:
Byung Chul SHIN
1
;
Hyun Ho RYU
;
Jong Hoon CHUNG
;
Byoung Rai LEE
;
Hyun Lee KIM
Author Information
1. Department of Internal Medicine, Seonam University College of Medicine, Gwangju, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Green Tea Extract;
Arginine;
Nitric Oxide;
Polyphenols
- MeSH:
Antioxidants/metabolism;
Arginine/metabolism/pharmacology/*toxicity;
Cell Line;
Cell Proliferation;
Cell Survival;
Flavonoids/metabolism;
Glomerular Mesangium/cytology/metabolism;
Humans;
Mesangial Cells/*cytology/metabolism;
Nitric Oxide/chemistry/metabolism;
Nitric Oxide Synthase Type II/metabolism;
Phenols/metabolism;
RNA, Messenger/metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Tea
- From:Journal of Korean Medical Science
2009;24(Suppl 1):S204-S209
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to investigate whether green tea extract (GTE) has the protective effects on excess L-arginine induced toxicity in human mesangial cell. Human mesangial cells treated with L-arginine were cultured on Dulbecco's modified eagle medium in the presence and absence of inducible nitric oxide synthase (iNOS) inhibitor and GTE. The cell proliferation was determined by 3 (4,5-dimethylthiazol- 2-yl)-2, 5-diphengltetrqzolium bromide, a tetrazole assay. The iNOS mRNA and its protein expression were detected by reverse transcription polymerase chain reaction and Western blot, respectively. The concentration of nitric oxide (NO) was measured by NO enzyme-linced immuno sorbent assay kit. L-arginine significantly inhibited the proliferation of human mesangial cells, and induced the secretion of NO to the media. NO production by L-arginine was significantly suppressed by GTE and iNOS inhibitor (p<0.01). The expression level of iNOS mRNA and its protein that was significantly increased by L-arginine was decreased by iNOS inhibitor but not by GTE. GTE protected the mesangial cells from the NO-mediated cytotoxicity by scavenging the NO rather than by iNOS gene expression. Therefore, we conclude that GTE has some protective effect for renal cells against oxidative injury possibly by polyphenols contained in GTE.
