Mechanism of HBx Protein Inhibiting DKK4 Expression and Its Effect on Proliferation and Migration of Hepatocarcinoma Cell Lines
10.3971/j.issn.1000-8578.2022.21.1524
- VernacularTitle:HBx蛋白抑制DKK4表达的机制及对肝癌细胞系增殖和迁移的影响
- Author:
Wenjing ZHANG
1
;
Gufen ZHANG
;
Xiaoyan WANG
;
Ying ZHANG
;
Huiqin QUAN
Author Information
1. Department of Infectious Diseases, Tangdu Hospital, Air Force Military Medical University, Xi'an 710038, China
- Publication Type:Research Article
- Keywords:
DKK4 protein;
Deacetylation;
HBx protein;
HCC
- From:
Cancer Research on Prevention and Treatment
2022;49(9):899-903
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of hepatitis B virus X protein down-regulating DKK4 and its effect on the proliferation, migration of HCC cell lines. Methods HCC cell lines HepG2 and SMMC7721 cells were infected with adenovirus encoding hepatitis B virus X protein (Ad-HBx), and GFP adenovirus (Ad-GFP) was designed as a control group. We used deacetylase inhibitor (TSA) to treat HCC cell lines and transfected HCC cell lines with small interfering RNA-histone deacetylase 1 (si-HDAC1) and lentivirus overexpressing DKK4. Western blot was used to detect the expression of DKK4, HDAC1 and SIRT1. The proliferation and migration ability of HCC lines were assessed using MTT, crystal violet experiment and Transwell experiment. Results DKK4 expression level was significantly downregulated after Ad-HBx infection (P < 0.05), and its expression level was recovered after TSA treatment (P < 0.05). After silencing HDAC1 with small interfering RNA, the expression of DKK4 could be restored (P < 0.05), the proliferation and migration of HDAC1-silencing or/and DKK4-overexpressing cells decreased (P < 0.05). Conclusion Hepatitis B virus X protein inhibits the expression of DKK4 protein by up-regulating HDAC1 and SIRT1. Silencing HDAC1 and over expressing DKK4 protein could inhibit the proliferation and migration of HCC cell lines infected with Ad-HBx.
