Effects of Circular RNA hsa_circ_0001922 on Proliferation, Migration and Invasion of Prostate Cancer Cells and Its Potential Molecular Mechanism
10.3971/j.issn.1000-8578.2022.21.1328
- VernacularTitle:环状RNA hsa_circ_0001922对前列腺癌细胞增殖、迁移和侵袭的影响及其潜在分子机制
- Author:
Yanyan ZHANG
1
;
Min ZHAO
;
Jing LIU
;
Hongyan GUO
;
Yinying HU
;
Lin ZHAO
;
Zhigang WANG
Author Information
1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanchang University, Nangchang 330000, China
- Publication Type:Research Article
- Keywords:
Prostate cancer;
hsa_circ_0001922;
Proliferation;
Invasion;
EMT
- From:
Cancer Research on Prevention and Treatment
2022;49(7):649-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of circular RNA hsa_circ_0001922 on the proliferation, migration and invasion of prostate cancer cell PC-3 and its underlying molecular mechanism. Methods qRT-PCR and RNA FISH were used to detect the expression level and localization of hsa_circ_0001922 in PC-3 cells respectively. After hsa_circ_0001922 was targeted inhibited, clone formation, Transwell assay and scratch assay were used to detect the proliferation, migration and invasion abilities of PC-3 cells. qRT-PCR and Western blot were used to detect the expression levels of EMT pathway-related molecules after inhibiting hsa_circ_0001922. Results The expression of circular RNA hsa_circ_0001922 was increased in PC-3 cells (P < 0.01) and it existed in the cytoplasm and nucleus. The invasion, migration and invasion abilities were significantly weakened (P < 0.05) after inhibiting hsa_circ_0001922; the expression of E-cadherin mRNA increased (P < 0.05) while the mRNA level of Vimentin decreased (P < 0.05). The results of Western blot were consistent with the above (both P < 0.05). Conclusion The circular RNA hsa_circ_0001922 may promote the proliferation, migration and invasion of PC-3 cells through the EMT pathway.
