Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1
10.3971/j.issn.1000-8578.2022.21.1202
- VernacularTitle:miR-581调控FOXO1对卵巢癌SKOV3细胞自噬的影响
- Author:
Haining GAO
1
;
Ruixia BAI
;
Pengwei ZHAO
;
Wanying SONG
;
Xuan LIN
Author Information
1. Inner Mongolia Medical College, Inner Mongolia Medical University, Hohhot 010000, China
- Publication Type:Research Article
- Keywords:
Ovarian cancer;
SKOV3 cells;
Autophagy;
miR-581;
FOXO1
- From:
Cancer Research on Prevention and Treatment
2022;49(5):403-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells. Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes. Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3 Ⅱ and Beclin1 (P < 0.01); miR-581 negatively regulated FOXO1 expression (P < 0.01) and FOXO1 promoted autophagy of SKOV3 cells (P < 0.01). Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1.
