Effect of IGF1Rβ Subunit Mutants on Proliferation, Migration and Apoptosis of Human Osteosarcoma Cells

VernacularTitle:IGF1受体的β亚基突变体对人骨肉瘤细胞增殖、迁移和凋亡的影响
Author: Zhongchi1 WEN ¹ ; Tuozhou1 LIU ; Hongbo HE ; Can ZHANG ; Yupeng LIU ; Zhan LIAO ; Liyi ZENG
Author Information

1. Department of Orthopedics, Xiangya Hospital Central South University, Changsha 410008, China
Publication Type:Research Article
Keywords: Insulin-like growth factor 1 receptor; Osteosarcoma; Mutant
From: Cancer Research on Prevention and Treatment 2022;49(5):390-395
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of IGF1R β subunit mutants sb-IGF1R and ma-IGF1R on the biological behavior of osteosarcoma 143B cells. Methods We designed and constructed sb-IGF1R and ma-IGF1R fragments. They were cloned into adenovirus AdEasy shuttle plasmid, to obtain Ad-sbIGF1R and Ad-maIGF1R. We observed the proliferation, migration and apoptosis of the osteosarcoma cells transfected with Ad-sbIGF1R, Ad-maIGF1R and Ad-IGF1R. The Ad-sbIGF1R, Ad-maIGF1R and Ad-GFP nude mouse models were constructed to evaluate the tumor growth in vitro. Results By plasmid PCR, IGF-1R β subunit mutant was overexpressed in osteosarcoma cells. Ad-sbIGF1R and Ad-maIGF1R significantly inhibited the proliferation and migration of osteosarcoma cells, and promoted cell apoptosis, and inhibited tumor growth in subcutaneous tumor-bearing nude mouse models. Conclusion IGF1R β subunit mutants inhibit the proliferation and migration of osteosarcoma cells and induce cell apoptosis.