Effect of RhoC Expression in Vascular Endothelial Cells on Proliferation and Invasion of Myeloma RPMI8226 Cells
10.3971/j.issn.1000-8578.2022.21.0934
- VernacularTitle:血管内皮细胞中RhoC的表达对骨髓瘤RPMI8226细胞增殖和侵袭的影响
- Author:
Miaomiao SUN
1
;
Kai LIU
;
Tong WANG
;
Sen QIU
;
Zhihua ZHAO
;
Kuisheng CHEN
Author Information
1. Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Henan Key Laboratory of Tumor Pathology, Zhengzhou 450052, China
- Publication Type:Research Article
- Keywords:
RhoC;
Myeloma;
Vascular endothelial cells;
Cell proliferation;
Invasion
- From:
Cancer Research on Prevention and Treatment
2022;49(4):299-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of RhoC expression in vascular endothelial cells on the proliferation and invasion of myeloma RPMI8226 cells and its possible mechanism. Methods RhoC shRNA lentivirus vector was constructed and transfected into myeloma vascular endothelial cells (MVECs) and human umbilical vein endothelial cells (HUVECs). The effects of conditioned medium on the proliferation, cell cycle and invasion of RPMI8226 cells were detected by CCK-8 test, flow cytometry and Transwell test. The expression of CDK, CyclinD1, AKT, PI3K, MMP2 and MMP9 were detected by Western blot. Results The expression of RhoC in MVECs and HUVECs were downregulated. The proliferation and invasion of RPMI8226 cells in RhoC shRNA group were lower than those in negative control group, and the cell cycle was blocked in G0/G1 phase (P < 0.05). The expressions of CDK, CyclinD1, AKT, PI3K, MMP2 and MMP9 in RhoC shRNA group were lower than those in negative control group (P < 0.05). Conclusion The expression of RhoC in MVECs can regulate the proliferation and invasion of myeloma RPMI8226 cells, and the mechanism may be related to the participation of CDK, CyclinD1, AKT, PI3K, MMP2 and MMP9.
