Mechanism of HSP90 Inhibitor AUY-922 Reversing ALK Inhibitor Resistance in Human Neuroblastoma Cells
10.3971/j.issn.1000-8578.2022.21.0949
- VernacularTitle:HSP90抑制剂AUY-922逆转人神经母细胞瘤细胞ALK抑制剂耐药的机制
- Author:
Yueqin WANG
1
;
Luyao FENG
;
Xin TIAN
Author Information
1. Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou 450052, China
- Publication Type:Research Article
- Keywords:
Neuroblastoma;
Drug resistance;
TAE684;
AUY-922;
Cell cycle arrest;
Signal pathway
- From:
Cancer Research on Prevention and Treatment
2022;49(4):294-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of HSP90 inhibitor AUY-922 reversing the resistance of human neuroblastoma cells to the second generation ALK inhibitor TAE684. Methods The effect of AUY-922 on the proliferation of human neuroblastoma cells SH-SY5Y and KELLY carrying ALK F1174L gene was detected by MTT method. The effect of AUY-922 on cell cycle of SH-SY5Y and KELLY cells was detected by flow cytometry. The effects of AUY-922 on cyclin Cdc2, ALK protein and downstream signal pathways p-Akt, p-Erk, p-stat3 in SH-SY5Y and KELLY cells were detected by Western blot. Results AUY-922 could significantly inhibit the proliferation of SH-SY5Y and KELLY cells, and the IC50 were about 0.30 μmol/L and 0.12 μmol/L, respectively. AUY-922 could induce cell cycle arrest of SH-SY5Y and KELLY cells in G2/M phase in a dose-dependent manner. AUY-922 could degrade Cdc2 and ALK proteins in SH-SY5Y and KELLY cells in a dose-dependent manner, and reduce the levels of p-Akt, p-Erk and p-stat3. Conclusion The reversal effect of HSP90 inhibitor AUY-922 on drug resistance of human neuroblastoma to ALK inhibitor may be related to its induction of G2/M cell cycle arrest, the degradation of ALK protein and the inhibition of downstream signal pathway.
