Differential effects of Ach and ATP on [Ca2+]c changes in mouse pancreatic β-cells
- VernacularTitle:Ach和ATP在小鼠胰岛β细胞诱导细胞内钙变化的比较
- Author:
Yufeng ZHAO
;
Yunlong ZHU
;
Jingjun ZHOU
;
Yuzhen HU
- Publication Type:Journal Article
- Keywords:
mouse, acetylcholine, adenosine triphosphate, pancreatic β-cells, intracellular Ca2+
- From:
Chinese Journal of Rehabilitation Theory and Practice
2003;9(9):545-547
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the differences between acetylcholine(Ach)-induced increase and adenosine triphosphate(ATP)-induced increase in cytosolic free Ca2+ concentration ([Ca2+]c) in mouse pancreatic β-cells. MethodsMouse pancreatic β-cells were primarily cultured and divided into two groups,one group was stimulated by Ach and another by ATP.[Ca2+]c was recorded with Fura-2 in normal condition, chelation of extracellular Ca2+ by EGTA and depletion of intracellular Ca2+ stores by Thapsigargin.ResultsAch induced a transient peak increase and sustained increase in [Ca2+]c. ATP induced a transient peak increase and no sustained increase. Chelation of extracellular Ca2+ by EGTA eliminated the sustained increase induced by Ach, and did not eliminate the transient peak increase induced by Ach and ATP. Depletion of intracellular Ca2+ stores by Thapsigargin eliminated the transient peak increase induced by Ach and ATP and the sustained increase induced by Ach. ConclusionsAch induces intracellular Ca2+ release and the following Ca2+ release-activated Ca2+ influx, and ATP induces intracellular Ca2+ release, but blocks the following Ca2+ release-activated Ca2+ influx.
