Research and exploration of salivary biological markers for hepatitis B-related hepatocellular carcinoma.
10.3760/cma.j.cn501113-20230210-00047
- VernacularTitle:乙型肝炎相关肝癌在唾液中生物学标志物的研究与探索
- Author:
Yan Hui LU
1
;
Qiu Yan LU
1
;
Zhi Yun YAN
1
;
Cheng Run XU
1
Author Information
1. Department of Infectious Diseases of People's Liberation Army, the 909th flospital, Dongnan Hospital of Xiamen University, Zhangzhou 363000, China.
- Publication Type:Journal Article
- Keywords:
Alpha-fetoprotein;
Biomarker;
Hepatocellular carcinoma;
Saliva;
α-1-acid glycoprotein 1
- MeSH:
Humans;
Carcinoma, Hepatocellular/metabolism*;
Liver Neoplasms/pathology*;
alpha-Fetoproteins/metabolism*;
Biomarkers;
Hepatitis B;
ROC Curve;
Glycoproteins;
Biomarkers, Tumor
- From:
Chinese Journal of Hepatology
2023;31(5):495-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study using isotope-labeled relative and absolute quantitative proteomics methodologies to screen for salivary biological markers as a simple, non-invasive tool for identifying hepatitis B-related HCC at an early stage. Methods: Saliva samples were collected to extract salivary proteins. Isotope-labeled relative and absolute quantitative proteomics were used to analyze the differentially expressed proteins between the hepatocellular carcinoma (HCC) and non-HCC groups. Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays were used to verify differential proteins and identify markers in liver cancer tissues and saliva. Statistical analysis was used to analyze the diagnostic efficiency of salivary biomarkers. Results: 152 differentially expressed salivary proteins were screened out between the HCC and non-HCC groups. Western blot, immunohistochemistry, and enzyme-linked immunosorbent assays validated that the expressions of α-1-acid glycoprotein 1 (ORM1) and alpha-fetoprotein (AFP) were significantly increased in HCC (P < 0.05). There was a significant correlation between salivary AFP and serum AFP (P < 0.05). HCC was diagnosed when salivary α-1-acid glycoprotein 1 combined with AFP. The area under the receiver operating characteristic curve was 0.8726 (95% confidence interval: 0.8104 ~ 0.9347), the sensitivity was 78.3%, and the specificity was 88%. Conclusion: Salivary AFP and α-1-acid glycoprotein 1 can serve as potential biomarkers for hepatitis B-related hepatocellular carcinoma.
