Effects of specimen preservation and transportation on blood paraquat concentration in rats.
10.3760/cma.j.cn121094-20221231-00613
- Author:
Dong Qi YAO
1
;
Han Yan LUO
1
;
Jian Xing HOU
1
;
Yi Qing SUN
1
;
Yan Ling DONG
1
;
Ying Ping TIAN
1
Author Information
1. Emergency Department of the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
- Publication Type:Journal Article
- Keywords:
Paraquat;
Poison detection;
Rat;
Specimen preservation;
Specimen transportation
- MeSH:
Rats;
Male;
Animals;
Rats, Sprague-Dawley;
Paraquat/pharmacology*;
Lung
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(6):447-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of duration, temperature and shake on paraquat (PQ) concentration in the blood of PQ-exposed rats during the specinen preservation and transportation. Methods: In March 2021, 60 SD male rats of Specific Pathogen Free class were randomly divided into low-dose group (10 mg/kg PQ) and high-dose group (80 mg/kg PQ). Each group was divided into 5 subgroups (normal temperature group, cold storage group, 37 ℃ storage group, shaking on normal temperature group and shaking on 37 ℃ group), six rats in each subgroup. The rats were given intraperitoneal injection of PQ, 1 h after exposure, the blood samples were obtained by cardiac extraction. After different interventions, the concentrations of PQ were detected and compared before and after the intervention in each subgroup. Results: In the shaking on 37 ℃ group, the results of PQ concentrations in PQ-exposed rats were significantly lower than those before the intervention (P<0.05). In the other subgroups, the results were not significantly different compared with before intervention (P>0.05) . Conclusion: The concentration of PQ in the blood of rats exposed to PQ was decreased by shaking for 4 hours at 37 ℃.
