The research of liver failure in Banna miniature pigs caused by amanita exitialis.
10.3760/cma.j.cn121094-20220531-00292
- VernacularTitle:致命鹅膏致版纳微型猪肝衰竭的研究
- Author:
Hong Yue XU
1
;
Jia Ju ZHONG
1
;
Qun Mei YAO
1
;
Lu LIU
1
;
Ya Guang HU
1
;
Cheng Min YU
1
Author Information
1. Department of Emergency Medicine, the People's Hospital of Chuxiong Yi Autonomous Prefecture, Chuxiong 675000, China.
- Publication Type:Journal Article
- Keywords:
Amanita;
Amanita exitialis;
Amanitins;
Banna miniature pig;
Liver failure;
Poisoning
- MeSH:
Animals;
Swine;
Amanitins/metabolism*;
Swine, Miniature/metabolism*;
Amanita/metabolism*;
Liver Failure, Acute;
Mushroom Poisoning/diagnosis*
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(6):443-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the characteristics of Banna miniature pig liver failure induced by amanita exitialis. Methods: From September to October 2020, a reverse high performance liquid chromatography (RP-HPLC) method was used to determine the toxin content of amanita exitialis solution, and 2.0 mg/kg amanita exitialis solution (α-amanitins+β-amanitins) was administered orally to Banna miniature pigs. Toxic symptoms, blood biochemical indexes and histopathological changes of liver, heart and kidney were observed at each time point. Results: All Banna miniature pigs died within 76 h of exposure, and different degrees of digestive tract symptoms such as nausea, vomiting and diarrhea appeared between 6 and 36 h. The biochemical indexes of alanine aminotransferase, aspartate aminotransferase, total bilirubin, lactate dehydrogenase, myoglobin, creatine kinase isoenzyme, blood urea nitrogen and creatinine increased significantly at 52 h after exposure, and the differences were statistically significant compared with 0 h (P<0.05). The bleeding of liver and heart was obvious under macroscopic and microscopic observation, hepatocyte necrosis, renal tubule epithelial cell swelling. Conclusion: Large dose of amanita exitialis can cause acute liver failure of Banna miniature pigs, which is in line with the pathophysiological characteristics of acute liver failure, and lays a foundation for further research on the toxic mechanism and detoxification drugs of amanita exitialis induced liver failure.
